KINETICS AND PATTERN OF ORGANELLE EXOCYTOSIS DURING TOXOPLASMA-GONDII HOST-CELL INTERACTION
KINETICS AND PATTERN OF ORGANELLE EXOCYTOSIS DURING TOXOPLASMA-GONDII HOST-CELL INTERACTION
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DOI：
10.1007/bf00931830
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发表时间：
1993-07-01
影响因子：
2
通讯作者：
JOINER, KA
中科院分区：
文献类型：
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作者：
DUBREMETZ, JF;ACHBAROU, A;JOINER, KA
用免疫荧光分析(IFA)和免疫电子显微镜观察感染后不同时期弓形虫致密颗粒(GRA2、GRA3)、棒状病毒(ROP1)和表面蛋白(SAG1)的去向。侵袭后几分钟内，速殖子顶端出现致密颗粒胞吐。通过连续切片和冷冻断裂研究，在同一生物体中同时发现了几个胞外事件。致密颗粒内容物首先被发现是被困在寄生虫和空泡膜之间的致密物质，然后空泡网络或空泡膜都可以用特定的抗体进行免疫标记。空泡网络被致密颗粒抗体强烈标记，但没有SAG1特异性探针标记，这表明该网络没有富含膜蛋白。除了强烈标记液泡膜外，GRA3抗体还标记了从寄生虫空泡延伸到宿主细胞细胞质的链。
The fate of Toxoplasma gondii dense-granule (GRA2, GRA3), rhoptry (ROP1), and surface (SAG1) proteins was followed by immunofluorescence assay (IFA) and immunoelectron microscopy at different stages after infection. Dense-granule exocytosis occurred in the apical area of the tachyzoite within minutes of invasion. Several exocytic events were found simultaneously in the same organism, both by serial sectioning and by freeze-fracture studies. Dense-granule contents were first found as a dense material trapped between parasite and vacuole membranes before either the vacuolar network or the vacuole membrane could be immunolabeled with specific antibodies. The vacuolar network was strongly labeled with dense-granule antibodies but not with the SAG1-specific probe, which suggests that the network is not enriched in membrane proteins. In addition to strongly labeling the vacuole membrane, GRA3 antibodies also labeled strands extending from the parasitophorous vacuoles into the host-cell cytoplasm.