Application of oxygen vectors to Claviceps purpurea cultivation
Application of oxygen vectors to Claviceps purpurea cultivation
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DOI：
10.1007/s002530100592
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发表时间：
2001-05-01
影响因子：
5
通讯作者：
Scheper, T
中科院分区：
文献类型：
--
作者：
Menge, M;Mukherjee, J;Scheper, T
介绍了两相发酵系统在紫果麦角菌产麦角肽生物碱中的应用。首次将全氟碳化合物(PFC)作为氧载体用于Clavicep发酵。在摇瓶培养中，PFC的加入使总生物碱的产量增加了五倍，药用重要成分麦角胺的产量增加了六倍。这一上升与添加的PFC的浓度不相关，人们认为这种增强是由于多种因素的组合，包括PFC的影响。在我们的研究中，其他氧载体，如几个碳氢化合物，被证明是很差的氧载体。PFC在生物反应器中的培养是可重复的，总生物碱和麦角胺的产量分别在第11天和第9天达到最大值。与摇瓶相比，生物反应器中总生物碱产量的增幅相对较小，这归因于反应器中的氧气利用能力不同。采用PFC工艺的运行优势是曝气量减少了五分之一。
The application of a two-phase fermentation system for the production of ergot peptide alkaloids by Claviceps purpurea is described. Perfluorocarbons (PFC) are used as oxygen vectors in Claviceps fermentation for the first time. In shake-flask cultivations, the inclusion of PFC in the medium brings about a five-fold increase in the total alkaloid production and a six-fold increase in the pharmaceutically important component, ergotamine. This rise cannot be correlated with the concentration of the added PFC and it is thought that the enhancement is due to a combination of factors, including the influence of PFC. Other oxygen vectors, such as several hydrocarbons, prove to be poor oxygen carriers in our study. Cultivations with PFC in a bioreactor are reproducible, the maximum total alkaloid and ergotamine production being attained on the 11th and 9th days, respectively. The relatively lower increase in the total alkaloid production in the bioreactor as compared to the shake-flasks is attributed to the unequal oxygen avail ability in the reactor. Processes with PFC offer the operational advantage of a five-fold reduction in aeration rate.