Small DNA circles as probes of DNA topology
Small DNA circles as probes of DNA topology
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DOI：
10.1042/bst20120320
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发表时间：
2013-04-01
影响因子：
3.9
通讯作者：
Maxwell, Anthony
中科院分区：
文献类型：
--
作者：
Bates, Andrew D.;Noy, Agnes;Maxwell, Anthony
小的DNA环可以在自然界中出现，例如蛋白质限制环，并且可以通过许多方法合成。这样的小圆圈为研究闭环DNA的结构、热力学和分子动力学提供了易于处理的系统。本文综述了DNA小环的产生、合成及其在研究DNA性质和DNA-蛋白质相互作用中的应用。特别是，我们强调使用原子模拟的小圆圈的分析。
Small DNA circles can occur in Nature, for example as protein-constrained loops, and can be synthesized by a number of methods. Such small circles provide tractable systems for the study of the structure, thermodynamics and molecular dynamics of closed-circular DNA. In the present article, we review the occurrence and synthesis of small DNA circles, and examine their utility in studying the properties of DNA and DNA-protein interactions. In particular, we highlight the analysis of small circles using atomistic simulations.