Functional mechanism study of the allelochemical myrigalone A identifies a group of ultrapotent inhibitors of ethylene biosynthesis in plants
Functional mechanism study of the allelochemical myrigalone A identifies a group of ultrapotent inhibitors of ethylene biosynthesis in plants
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DOI：
10.1016/j.xplc.2024.100846
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发表时间：
2024-06-10
影响因子：
10.5
通讯作者：
Williams，Robin S. B.
中科院分区：
文献类型：
--
作者：
Heslop-Harrison，George;Nakabayashi，Kazumi;Williams，Robin S. B.
化感物质是植物释放的一类天然产物，如根、叶和果实分泌物，它们干扰邻近植物的生长和生存。了解化感物质如何调节植物的反应，可能会为更好地控制植物功能提供有价值的新途径。杨梅产生的一种化感物质Myrigone A(Mya)通过一种未知的机制抑制种子萌发和幼苗生长。在这里，我们使用易处理的模型Dictyostelialdiscoideum来研究Mya，发现它的活性依赖于植物乙烯合成蛋白1-氨基环丙烷-1-羧酸氧化酶(ACO)的保守同源物。此外，在硅模塑预测MYA与催化口袋内的ACO的直接结合。在盘状茎中，Aco的消融模拟了Mya依赖的发育延迟，而Mya可以部分地被外源乙烯恢复，Mya减少了乙烯的产生。在拟南芥中，MYA处理延迟了种子的萌发，这一效应被外源乙烯拯救。它还模仿现有的ACO抑制剂对根和下胚轴延伸的影响，阻止依赖乙烯的根毛产生，并减少乙烯的产生。最后，在硅结合分析中，确定了一系列高效的乙烯抑制剂，它们可以阻断拟南芥对乙烯的依赖反应，并减少乙烯的产生。因此，我们证明了化感物质MYA减少乙烯生物合成的分子机制，并确定了一系列乙烯调节反应的超强抑制物。
Allelochemicals represent a class of natural products released by plants as root, leaf, and fruit exudates that interfere with the growth and survival of neighboring plants. Understanding how allelochemicals function to regulate plant responses may provide valuable new approaches to better control plant function. One such allelochemical, Myrigalone A (MyA) produced byMyrica gale, inhibits seed germination and seedling growth through an unknown mechanism. Here, we investigate MyA using the tractable modelDictyostelium discoideumand reveal that its activity depends on the conserved homolog of the plant ethylene synthesis protein 1-aminocyclopropane-1-carboxylic acid oxidase (ACO). Furthermore,in silicomodeling predicts the direct binding of MyA to ACO within the catalytic pocket. InD.discoideum, ablation ofACOmimics the MyA-dependent developmental delay, which is partially restored by exogenous ethylene, and MyA reduces ethylene production. InArabidopsis thaliana, MyA treatment delays seed germination, and this effect is rescued by exogenous ethylene. It also mimics the effect of established ACO inhibitors on root and hypocotyl extension, blocks ethylene-dependent root hair production, and reduces ethylene production. Finally,in silicobinding analyses identify a range of highly potent ethylene inhibitors that block ethylene-dependent response and reduce ethylene production inArabidopsis. Thus, we demonstrate a molecular mechanism by which the allelochemical MyA reduces ethylene biosynthesis and identify a range of ultrapotent inhibitors of ethylene-regulated responses.