Characterization of less pathogenic infectious molecular clones derived from acute-pathogenic SHIV-89.6P stock virus
Characterization of less pathogenic infectious molecular clones derived from acute-pathogenic SHIV-89.6P stock virus
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DOI：
10.1006/viro.2000.0839
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发表时间：
2001-03-30
期刊：
影响因子：
3.7
通讯作者：
Miura, T
中科院分区：
文献类型：
--
作者：
Kozyrev, IL;Ibuki, K;Miura, T
为了更好地了解SHIV-89.6P毒株的急性致病性。其在猴中接种后一个月内诱导显著的CD 4细胞损失，我们构建了四个感染性分子克隆（cl 18、cl 64、cl 69和cl 71）。与亲本病毒一样，CI 64、cl 69和cl 71在猴CD 4（+）细胞系中显示出高体外复制能力和致病样效应（CD 4下调），而cl 18显示出较低的复制能力，不能下调CD 4。将具有与亲本病毒相似的体外特性的Ci 64接种于4只恒河猴。所有猴的血浆病毒载量与亲本病毒相似，在接种后2周达到峰值。然而，病毒载量逐渐下降，病毒未能在受感染的猴子中引起艾滋病样疾病，但它诱导了强烈的抗病毒抗体反应。这些结果证明了亲本SHIV-89.6P病毒原种的多克隆性质，并证明了cl 64除了其高复制性之外，可能与亲本病毒在性质上不同。(C)北京：科学出版社.
For a better understanding of the acute pathogenicity of SHIV-89.6P stock virus. which induces prominent CD4 cell loss within a month after inoculation in monkeys, we have constructed four infectious molecular clones (cl 18, cl 64, cl 69, and cl 71). CI 64, cl 69, and cl 71, like the parental virus, showed a high in vitro replication ability and a pathogenic-like effect (CD4 downmodulation) in a monkey CD4(+) cell line, whereas cl 18 showed a lower replication ability and could not downmodulate CD4. Ci 64, which has characteristics similar to those of the parental virus in vitro, was inoculated into four rhesus monkeys. All monkeys showed a plasma viral load similar to that of the parental virus with a peak at 2 weeks after inoculation. However, the viral load gradually decreased and the virus failed to cause an AIDS-like disease in infected monkeys, but it induced a strong antiviral antibody response. These results demonstrate the polyclonal nature of the parental SHIV-89.6P virus stock and demonstrate that cl 64, aside from its high replicability, may differ qualitatively from the parental virus. (C) 2001 Academic Press.