Inhibitory Effects of Sulfated Polysaccharides from the Sea Cucumber Cucumaria Frondosa against A beta 40 Aggregation and Cytotoxicity
Inhibitory Effects of Sulfated Polysaccharides from the Sea Cucumber Cucumaria Frondosa against A beta 40 Aggregation and Cytotoxicity
复制标题
海参硫酸多糖对 Aβ40 聚集和细胞毒性的抑制作用
DOI：
10.1021/acschemneuro.1c00223
复制
发表时间：
2021
影响因子：
5
通讯作者：
Jia Lee
中科院分区：
文献类型：
--
作者：
Li Gao;Zhou Yu;Yang Wu-Yue;Zhang Chen;Hong Liu;Jia Lee
Aβ的异常聚集和沉积是阿尔茨海默病的致病因素之一。Aβ聚集抑制剂的开发被认为是预防和治疗阿尔茨海默病的一种可能的方法。食用海参含有许多生物活性分子，包括皂苷、磷脂、多肽和多糖。在此，我们报道了从海参中提取的多糖可以降低Aβ40的聚集性和细胞毒性。利用多种生化和生物物理手段，我们发现该多糖对Aβ40的聚集有抑制作用。化学动力学分析进一步表明，多糖的主要抑制作用是通过分解成熟的纤维来实现的，这反过来又降低了Aβ的细胞毒性。这些结果表明，从海参中提取的多糖可以作为A-β的有效抑制剂。
Abnormal aggregation and deposition of Aβ is one of the causative agents for Alzheimer’s disease. The development of inhibitors for Aβ aggregation has been considered a possible method to prevent and treat Alzheimer’s disease. Edible sea cucumbers contain many bioactive molecules, including saponins, phospholipids, peptides, and polysaccharides. Herein, we report that polysaccharides extracted from sea cucumberCucumaria frondosacould reduce the aggregation and cytotoxicity of Aβ40. By utilizing multiple biochemical and biophysical instruments, we found that the polysaccharides could inhibit the aggregation of Aβ40. A chemical kinetics analysis further suggested that the major inhibitory effects of the polysaccharides were achieved by disassembling mature fibrils, which in turn reduced the cytotoxicity of Aβ. These results suggested that the polysaccharides extracted from sea cucumber could be used as an effective inhibitor for Aβ.