The influence of rigid or flexible linkage between two ligands on the effective affinity and avidity for reversible interactions with bivalent receptors
The influence of rigid or flexible linkage between two ligands on the effective affinity and avidity for reversible interactions with bivalent receptors
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DOI：
10.1002/jmr.836
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发表时间：
2007-07-01
影响因子：
2.7
通讯作者：
Bobrovnik, S. A.
中科院分区：
文献类型：
--
作者：
Bobrovnik, S. A.
配体和受体之间的二价或多价协同结合比同一配体的单价结合更加紧密和有效。正因为如此，许多生物过程都涉及到实现调控机制的多价结合。因此，有必要发展一种通用的形式来预测每个配体亚基的结合亲和力、它们之间的柔性连接子的长度和相互作用的亲和力之间的关系。在这里，我们考虑一种基于对代数方程系统的单价和多价配体-受体结合反应的平衡状态的描述的方法。这种方法可以评估二价结合的亲和力，并确定在平衡状态下将获得的配体-受体复合体的浓度。本文的分析可用于分析二价受体和由刚性或柔性连接物共价连接的两个亚基组成的配体的结合行为。版权所有(C)2007 John Wiley&Sons，Ltd.
Bivalent or polyvalent cooperative binding between ligand and receptor is much tighter and more efficient than monovalent binding of the same counterparts. Because of this, many biological processes involve polyvalent binding for realization of regulatory mechanisms. For this reason it is necessary to develop a general formalism for prediction of the relationship between the binding affinities of each ligand subunits, the length of the flexible linker between them and avidity of the interaction. Here, we consider an approach that is based on the description of the state of equilibrium for the reaction of mono- and multivalent ligand-receptor binding of algebraic equation systems. This approach allows the evaluation of the avidity of bivalent binding and to determine the concentrations of ligand-receptor complexes, which will be obtained at the equilibrium state. The analysis presented here may be useful in analysing the binding behaviour of a bivalent receptor and a ligand consisting of two subunits covalently connected with a rigid or flexible linker. Copyright (C) 2007 John Wiley & Sons, Ltd.