Functional and physical characteristics of rat Leydig cell populations isolated by metrizamide and Percoll gradient centrifugation.
Functional and physical characteristics of rat Leydig cell populations isolated by metrizamide and Percoll gradient centrifugation.
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DOI：
10.1095/biolreprod37.2.335
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发表时间：
1987-09
影响因子：
3.6
通讯作者：
M. Georgiou;A. Payne
中科院分区：
文献类型：
--
作者：
M. Georgiou;A. Payne
比较了睾丸细胞在两种不同密度梯度培养基Percoll和Metrizamide中离心获得的间质细胞群的物理和功能特性。percoll梯度离心得到两个间质细胞带（峰I和峰II），其密度和睾酮产生能力与在甲咪唑胺梯度中分离的各自的间质细胞带（种群I和种群II）相当。无论采用何种梯度分离，密度较大的间质细胞带（II）比密度较小的间质细胞带(I)产生睾酮的能力更大。甲咪唑胺梯度离心使大多数生殖细胞与“轻”（种群I）间质细胞分离，而在Percoll梯度下，生殖细胞与峰值I间质细胞共存。Percoll梯度对间质细胞的分离高度依赖于培养基中Ca2+和Mg2+的存在，而这些阳离子对甲咪唑胺对间质细胞的分离没有影响。综上所述，甲咪唑胺梯度离心分离得到两个间质细胞群体，其功能和物理性质与Percoll梯度分离的群体相似。
The physical and functional properties of Leydig cell populations obtained by centrifugation of testicular cells in two different density gradient media, Percoll and Metrizamide, were compared. Percoll-gradient centrifugation yielded two Leydig cell bands (Peak I and Peak II) that were comparable, as to their density and testosterone-producing capacity, to the respective Leydig cell bands, Population I and Population II, isolated in a Metrizamide gradient. The denser Leydig cell band (II) had a greater capacity for testosterone production than the less dense band (I), regardless of the type of gradient used for its isolation. Metrizamide gradient centrifugation separated the majority of germ cells from the "light" (Population I) Leydig cells, whereas in the Percoll gradient, germ cells comigrated with Peak I Leydig cells. Leydig cell separation by Percoll gradients was highly dependent on the presence of Ca2+ and Mg2+ in the medium, while these cations had no effect on the separation of Leydig cells by Metrizamide. In conclusion, Metrizamide gradient centrifugation yielded two Leydig cell populations of similar functional and physical properties to the respective populations isolated in Percoll gradients.