Point-of-care method for total white cell count: an evaluation of the HemoCue WBC device
Point-of-care method for total white cell count: an evaluation of the HemoCue WBC device
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DOI：
10.1111/j.1751-553x.2008.01093.x
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发表时间：
2009-12-01
影响因子：
3
通讯作者：
Lewis, S. M.
中科院分区：
文献类型：
--
作者：
Osei-Bimpong, A.;Jury, C.;Lewis, S. M.
即时检验（POCT）正在成为血液学实验室实践的重要辅助手段。血细胞计数的一个重要组成部分是总白色细胞计数（WBC）。以前，这需要费力的显微镜细胞计数，但现在可以通过自动化进行;然而，在许多资源不足的国家，昂贵的自动计数器只在极少数中心医院可用。此外，这两种方法在大多数POCT情况下都不实用。HemoCue WBC是一种简化的替代方法，由预装试剂的一次性比色皿和基本的图像分析技术组成。本报告描述了对其效用的评估。同时用HemoCue WBC分析仪和参考分析仪对本院500份常规血标本的WBC进行平行检测，以评价HemoCue WBC分析仪的准确性和实用性。检测内容包括精密度、线性、血液样本类型、抗凝剂和血液样本中的潜在干扰物质。在准确度测试中，200个样本中有192个样本与NEQAS参考样本的百分比差异< 10%，而其余8个样本的差异<12%，因此符合临床实验室改进修正案（CLIA）-88法规的要求。在用潜在干扰物质检测的样本中，只有那些具有> 2%正常红细胞或网织红细胞增多症的样本显示出与参考测量值的显著差异。HemoCue WBC在0.4-30.0 x 109/l的分析范围内对于WBC计数是可靠的，除非样本中有大量的正常红细胞或网织红细胞。它使用简单，为血液学POCT提供了一个有价值的进步。
P>Point-of-care testing (POCT) is becoming an important adjunct to haematology laboratory practice. An important component of the blood count is the total white cell count (WBC). Previously, this required laborious microscopic cell counting, but it can now be performed by means of automation; however, in many under-resourced countries, costly automated counters are only available in very few central hospitals. Moreover, neither method is practical in most POCT situations. The HemoCue WBC has been developed as a simplified alternative method, consisting of a reagent pre-loaded disposable cuvette together with basic image analysis technology. This report describes an assessment of its utility. The WBC of 500 routine blood samples from the hospital were tested in parallel by the HemoCue WBC and by a reference analyser to assess accuracy and utility of the former. The tests included precision, linearity, type of blood sample and anticoagulant and potential interfering substances in blood specimens. In the tests for accuracy, 192 of the 200 showed percentage difference from the NEQAS reference of < 10% whilst the remaining eight samples differed by < 12%, thus meeting the requirements of Clinical laboratory improvement amendments (CLIA)-88 regulations. Of the samples tested with potential interfering substances only those with > 2% normoblasts or reticulocytosis showed significant differences from the reference measurements. The HemoCue WBC is reliable for WBC counts within the analytical range of 0.4-30.0 x 109/l, except in samples where there are significant numbers of normoblasts or reticulocytes. It is simple to use and provides a valuable advance in the facilities available for POCT in haematology.