Branch specific and spike-order specific action potential invasion in basal, oblique, and apical dendrites of cortical pyramidal neurons.
Branch specific and spike-order specific action potential invasion in basal, oblique, and apical dendrites of cortical pyramidal neurons.
复制标题
皮质锥体神经元的基底树突、斜树突和顶树突中的分支特异性和尖峰顺序特异性动作电位入侵。
DOI：
10.1117/1.nph.2.2.021006
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发表时间：
2015
期刊：
影响因子：
5.3
通讯作者：
Antic,SrdjanD
中科院分区：
文献类型：
--
作者：
Zhou,Wen-Liang;Short,ShainaM;Rich,MatthewT;Oikonomou,KaterinaD;Singh,MandakiniB;Sterjanaj,EnasV;Antic,SrdjanD
在新皮质锥体神经元中，动作电位（AP）从轴突传播到树突树以影响远端突触。传统上，AP反向传播研究在厚的顶端树干。在这里，我们使用科恩开发的光学成像的原则，以调查AP侵入薄树突状分支（基底，斜，和簇）的前额叶皮层L5锥体神经元。来自相邻树突的多位点光学记录揭示了属于同一细胞的两个看似相等的树突分支（“姐妹分支”）之间的明显二分法。我们通过揭示AP电压波形，记录了基底支和斜支AP侵袭的可变疗效。使用快速多位点钙成像，我们发现，列车的AP过滤不同的两个顶端簇分支之间。虽然一个树突分支通过AP序列中的所有尖峰，但属于同一神经元、同一皮层和距细胞体相同路径距离的另一个分支仅经历一个尖峰。我们的数据表明，树突电压和钙瞬变的巨大差异，检测锥体神经元的树突中，从A型电导率的不均匀分布，AP传播的路径中的分支点的聚集数量和树突直径的微小差异引起。
In neocortical pyramidal neurons, action potentials (APs) propagate from the axon into the dendritic tree to influence distal synapses. Traditionally, AP backpropagation was studied in the thick apical trunk. Here, we used the principles of optical imaging developed by Cohen to investigate AP invasion into thin dendritic branches (basal, oblique, and tuft) of prefrontal cortical L5 pyramidal neurons. Multisite optical recordings from neighboring dendrites revealed a clear dichotomy between two seemingly equal dendritic branches belonging to the same cell (“sister branches”). We documented the variable efficacy of AP invasion in basal and oblique branches by revealing their AP voltage waveforms. Using fast multisite calcium imaging, we found that trains of APs are filtered differently between two apical tuft branches. Although one dendritic branch passes all spikes in an AP train, another branch belonging to the same neuron, same cortical layer, and same path distance from the cell body, experiences only one spike. Our data indicate that the vast differences in dendritic voltage and calcium transients, detected in dendrites of pyramidal neurons, arise from a nonuniform distribution of A-typeconductance, an aggregate number of branch points in the path of the AP propagation and minute differences in dendritic diameter.