Serodiagnosis of human bocavirus infection
Serodiagnosis of human bocavirus infection
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DOI：
10.1086/526532
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发表时间：
2008-02-15
影响因子：
11.8
通讯作者：
Soderlund-Venermo, Maria
中科院分区：
文献类型：
--
作者：
Kantola, Kalle;Hedman, Lea;Soderlund-Venermo, Maria
背景。最近发现了一种新的人类致病性细小病毒——人博卡病毒（HBoV），它与幼儿呼吸道疾病有关。然而，许多患者表现出低病毒DNA载量，这表明HBoV的持久性和基于聚合酶链反应的诊断存在问题。此外，对HBoV的免疫力一无所知。我们检查了hbov特异性全身B细胞反应，并评估了它们在患有呼吸道疾病的幼儿中的诊断用途。患者和方法。本文采用2种重组HBoV衣壳抗原（病毒蛋白1和病毒蛋白2的独特部分）对117例急性喘息患儿血清进行免疫印迹检测。病毒蛋白2在免疫反应性方面优于病毒蛋白1的独特部分。根据病毒蛋白2测定，49例HBoV聚合酶链反应阳性的儿童中有24例（49%）有免疫球蛋白（Ig） M抗体，36例（73%）有IgG抗体，29例（59%）有IgM抗体和/或IgG抗体水平升高。在22例抗体水平升高的患者中，20例（91%）鼻咽部HBoV DNA高负荷，支持高HBoV DNA负荷表明急性原发性感染的假设，而低负荷似乎不太具有临床意义。在先前确定为急性HBoV感染的患者亚组中（定义为鼻咽部病毒载量高、病毒血症和无其他病毒感染），9例患者中有9例（100%）有血清学证据表明原发感染。在68名鼻咽部聚合酶链反应结果为HBoV阴性的喘息儿童的对照组中，9名（13%）有IgM抗体，包括5名IgG抗体水平升高和病毒血症。与人细小病毒B19无交叉反应。HBoV引起的呼吸道感染是全身性的，可引起B细胞免疫反应，可通过血清学诊断。血清学诊断与鼻咽部高病毒载量和病毒血症相关。血清学检测是揭示HBoV感染与疾病之间关系的准确工具。
Background. A new human-pathogenic parvovirus, human bocavirus (HBoV), has recently been discovered and associated with respiratory disease in small children. However, many patients have presented with low viral DNA loads, suggesting HBoV persistence and rendering polymerase chain reaction-based diagnosis problematic. Moreover, nothing is known of HBoV immunity. We examined HBoV-specific systemic B cell responses and assessed their diagnostic use in young children with respiratory disease.Patients and methods. Paired serum samples from 117 children with acute wheezing, previously studied for 16 respiratory viruses, were tested by immunoblot assays using 2 recombinant HBoV capsid antigens: the unique part of virus protein 1 and virus protein 2.Results. Virus protein 2 was superior to the unique part of virus protein 1 with respect to immunoreactivity. According to the virus protein 2 assay, 24 (49%) of 49 children who were positive for HBoV according to polymerase chain reaction had immunoglobulin (Ig) M antibodies, 36 (73%) had IgG antibodies, and 29 (59%) exhibited IgM antibodies and/or an increase in IgG antibody level. Of 22 patients with an increase in antibody levels, 20 (91%) had a high load of HBoV DNA in the nasopharynx, supporting the hypothesis that a high HBoV DNA load indicates acute primary infection, whereas a low load seems to be of less clinical significance. In a subgroup of patients who were previously determined to have acute HBoV infection (defined as a high virus load in the nasopharynx, viremia, and absence of other viral infections), 9 (100%) of 9 patients had serological evidence of primary infection. In the control group of 68 children with wheezing who had polymerase chain reaction results negative for HBoV in the nasopharynx, 9 (13%) had IgM antibodies, including 5 who displayed an increase in IgG antibody levels and were viremic. No cross-reactivity with human parvovirus B19 was detected.Conclusions. Respiratory infections due to HBoV are systemic, elicit B cell immune responses, and can be diagnosed serologically. Serological diagnoses correlate with high virus loads in the nasopharynx and with viremia. Serological testing is an accurate tool for disclosing the association of HBoV infection with disease.