Comprehensive Proteomic Analysis of PGC7-Interacting Proteins
Comprehensive Proteomic Analysis of PGC7-Interacting Proteins
复制标题
PGC7 相互作用蛋白的综合蛋白质组学分析
DOI：
10.1021/acs.jproteome.6b00883
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发表时间：
2017-09-01
影响因子：
4.4
通讯作者：
Guo, Zekun
中科院分区：
文献类型：
--
作者：
Liu, Hongliang;Zhang, Lei;Guo, Zekun
Primordial germ cell 7 (PGC7), a maternal factor essential for early development, plays a critical role in the regulation of DNA methylation, transcriptional repression, chromatin condensation, and cell division and the maintenance of cell pluripotentiality. Despite the fundamental roles of PGC7 in these cellular processes, only a few molecular and functional interactions of PGC7 have been reported. Here, a streptavidin biotin affinity purification technique combined with LC-MS/MS was used to analyze potential proteins that interact with PGC7. In total, 291 potential PGC7-interacting proteins were identified. Through an in-depth bioinformatic analysis of potential interactors, we linked PGC7 to critical cellular processes including translation, RNA processing, cell cycle, and regulation of heterochromatin structure. To better understand the functional interactions of PGC7 with its potential interactors, we constructed a protein-protein interaction network using the STRING database. In addition, we discussed in detail the interactions between PGC7 and some of its newly validated partners. The identification of these potential interactors of PGC7 expands our knowledge on the PGC7 interactome and provides a valuable resource for understanding the diverse functions of this protein.
