The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays
The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays
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DOI：
10.1016/j.jcv.2012.04.010
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发表时间：
2012-07-01
影响因子：
8.8
通讯作者：
Obermeier, Martin
中科院分区：
文献类型：
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作者：
Verheyen, Jens;Neumann-Fraune, Maria;Obermeier, Martin
背景资料：乙型肝炎表面抗原（hepatitis-B surface antigen，HBsAg）的定量检测有助于鉴别慢性HBV感染患者中的非活性携带者，并预测干扰素治疗的结果。目的：比较两种定量HBsAg检测方法在检测体外表达的HBsAg突变体方面的效果。在HuH 7细胞中表达HBsAg突变体（n = 35），并在两种不同的定量HBsAg测定中测试上清液结果：两种检测方法均能检测到所有HBsAg突变体，但Architect系统的检测结果总体上高于Elecsys系统。与野生型相比，使用两种检测方法检测HBsAg突变体的结果相似。然而，HBsAg突变T123 A是根据定量的架构师，而HBsAg突变P142 L，P142 S和G145 K产生较低的结果在Elecsys system.Conclusions：即使HBsAg检测优化了HBsAg突变体的检测，不一致的结果，获得了一些HBsAg突变体在两个定量HBsAg检测。当用两种不同的定量HBsAg检测试剂盒检测同一患者的样本时，必须考虑这些结果。(C)2012年爱思唯尔B。版权所有© 2016
Background: The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome.Objective: To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro.Study design: HBsAg mutants (n = 35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche).Results: All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system.Conclusions: Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays. (C) 2012 Elsevier B. V. All rights reserved.