CD43 (leukosialin, sialophorin, large sialoglycoprotein) can be expressed in both normal and Wiskott-Aldrich fibroblasts via transfection of a leukosialin cDNA.
CD43 (leukosialin, sialophorin, large sialoglycoprotein) can be expressed in both normal and Wiskott-Aldrich fibroblasts via transfection of a leukosialin cDNA.
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通过转染白唾液酸蛋白 cDNA，CD43（白唾液酸蛋白、唾液酸蛋白、大唾液酸糖蛋白）可在正常成纤维细胞和 Wiskott-Aldrich 成纤维细胞中表达。
DOI：
10.1002/eji.1830200703
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发表时间：
1990
影响因子：
5.4
通讯作者：
Frelinger,JG
中科院分区：
文献类型：
--
作者：
Pallant,A;Fukuda,M;Frelinger,JG
人白唾液酸蛋白是在淋巴造血系统细胞表面发现的最丰富的唾液酸糖蛋白之一。Leukosialin，也称为唾液酸蛋白，参与T细胞增殖，其分子同种型在细胞活化时发生变化。我们发现，人白唾液酸蛋白与单克隆抗体（mAb）G10 - 2、G19 - 1（CD 43）和B1 B6（大唾液酸糖蛋白）描述的抗原相同。转化细胞裂解物的免疫印迹分析表明了这种同一性。此外，用人leukosialin cDNA转染的成纤维细胞获得了对这些mAb的反应性，这最终表明这些mAb识别的分子由相同的cDNA决定。在转染的人和小鼠成纤维细胞的表面上容易检测到白唾液酸蛋白基因的表达。转染子的免疫印迹分析表明，人类蛋白质的加工发生在两个物种中。Wiskott-Aldrich综合征（WAS）是一种X染色体连锁免疫缺陷疾病，据报道，患者的白唾液酸蛋白表达发生了变化。虽然基本上所有来自正常个体的转染的肿瘤和原代成纤维细胞在细胞表面上表达转染的基因，但只有一半的转染的Wiskott-Aldrich成纤维细胞表达CD 43。尽管如此，正常和WAS转染的成纤维细胞的免疫印迹分析的抗原模式似乎相同。这些结果表明，WAS衍生的细胞可以表达leukosialin，并且WAS X染色体突变的产物可能不在成纤维细胞中表达。
Human leukosialin is among the most abundant sialoglycoproteins found on the surface of cells of the lympho‐hematopoietic system. Leukosialin, also known as sialophorin, is involved in T cell proliferation, and its molecular isoform changes upon cellular activation. We show that human leukosialin is identical to the antigens described by the monoclonal antibodies (mAb) G10‐2, G19‐1 (CD43) and B1B6 (large sialoglycoprotein). This identity was suggested by immunoblot analysis of transformed cell lysates. Further, fibroblasts transfected with the human leukosialin cDNA gain reactivity to these mAb, showing conclusively that molecules recognized by these mAb are determined by the same cDNA. Expression of the leukosialin gene is readily detected on the surface of transfected human and mouse fibroblasts. Immunoblot analysis of the transfectants indicates that processing of the human protein occurs in both species. Alterations of leukosialin expression have been reported in patients with the Wiskott‐Aldrich Syndrome (WAS), an X‐chromosome‐linked immunodeficiency disease. While essentially all of the transfected tumor and primary fibroblasts from normal individuals express the transfected gene on the cell surface, only half of the transfected Wiskott‐Aldrich fibroblasts express CD43. Nonetheless, the antigenic pattern by immunoblot analysis of both normal and WAS‐transfected fibroblasts appears identical. These results indicate that WAS‐derived cells can express leukosialin and that the product of WAS X‐chromosome mutation may not be expressed in fibroblasts.