Localization of human interleukin 13 receptor in non-haematopoietic cells
Localization of human interleukin 13 receptor in non-haematopoietic cells
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DOI：
10.1006/cyto.2000.0814
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发表时间：
2001-01-21
期刊：
影响因子：
3.8
通讯作者：
Izuhara, K
中科院分区：
文献类型：
--
作者：
Akaiwa, M;Yu, B;Izuhara, K
尽管白细胞介素（IL-）13在造血细胞中的功能作用已经得到了很好的研究，但在非造血细胞中的功能作用仍有待研究。IL-13通过与靶细胞上IL-13受体（IL-13R）结合发挥作用，靶细胞由IL-13R α 1和IL-4受体α链（IL-4R α）组成，但IL-13R在各组织中的定位研究尚未见报道。为了解决这个问题，我们制备了抗il - 13r α 1单克隆抗体，并使用该抗体和抗il - 4r α抗体进行免疫组化。这两种成分在所有检查组织中的分布是相同的。皮肤角质形成细胞、毛囊、皮脂腺和汗腺染色阳性；鼻组织中纤毛呼吸道上皮细胞；在心肌细胞中；在胃中央凹细胞、胃腺和平滑肌层；在肝脏的肝细胞中。然而，脑和骨髓未见染色。部分组织中可见成纤维细胞和内皮细胞染色。这些结果为阐明IL-13在特应性皮炎和变应性鼻炎中已知的病理作用以及未知的生理作用提供了线索。(C) 2001学术出版社。
Although the functional roles of interleukin (IL-)13 in haematopoietic cells are well investigated, those in non-haematopoietic cells remain to be addressed. IL-13 exerts its actions by binding to the IL-13 receptor (IL-13R) on target cells, which is composed of IL-13R alpha1 and the IL-4 receptor alpha chain (IL-4R alpha), However, there has been no study of localization of IL-13R in each tissue. To address this question, we generated monoclonal anti-IL-13R alpha1 antibody, and performed immunohistochemistry using this antibody and anti-IL-4R alpha antibody. Distribution of these two components was the same in all examined tissues. Staining was positive in keratinocytes, hair follicles, and sebaceous and sweat glands in skin; in ciliated respiratory epithelial cells in nasal tissue; in heart muscle cells; in foveola cells, gastric glands, and the smooth muscle layer in stomach; and in hepatocytes in liver. However, staining was undetectable in brain and bone marrow. Fibroblasts and endothelial cells were stained in some tissues. These results provide clues to elucidate the known pathological roles of IL-13 in atopic dermatitis and allergic rhinitis, as well as its unknown physiological roles. (C) 2001 Academic Press.