Molecular detection of potentially toxic cyanobacteria and their associated bacteria in lake water column and sediment
Molecular detection of potentially toxic cyanobacteria and their associated bacteria in lake water column and sediment
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DOI：
10.1007/s11274-010-0322-x
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发表时间：
2010-08-01
影响因子：
4.1
通讯作者：
Neofitou, Christos
中科院分区：
文献类型：
--
作者：
Kormas, Konstantinos Ar.;Vardaka, Elisabeth;Neofitou, Christos
我们研究了蓝藻和细菌的分子多样性在一个湖的水开花与有毒的蓝藻长历史（湖Kastoria，希腊）。我们还测试了形成水华的蓝藻是否在水华2年后保存在湖泊沉积物中的假设。藻华期间的优势蓝藻包括可能产生毒素的铜绿微囊藻和与微囊藻属密切相关的其他几种藻球菌形式。这表明，使用特定的蓝藻引物似乎是非常有益的描述蓝藻在水华期间。细菌群落表现出高度的多样性，主要由单型和双型组成。大多数种型为典型的湖泊细菌，包括一些潜在的病原体和毒素代谢细菌，表明优势毒性蓝藻对细菌群落结构没有显著影响。在沉积物中，在水华发生2年后，没有检索到形成水华的蓝藻，这表明它们不能保存在沉积物中。与水柱相似，沉积物的细菌多样性也很高，主要由尚未培养的细菌组成，这些细菌与有机物降解发生的环境有关。
We investigated the molecular diversity of cyanobacteria and bacteria during a water bloom in a lake with a long history of toxic cyanobacterial blooms (Lake Kastoria, Greece). We also tested the hypothesis whether bloom-forming cyanobacteria are preserved in the lake's sediment 2 years after the bloom. The dominant cyanobacteria during the bloom included the potentially toxin-producing Microcystis aeruginosa and several other Chroococcales forms closely related to the genus Microcystis. This suggests that the use of cyanobacterial-specific primers seems to be very informative in describing the cyanobacteria during the water blooms. The bacterial community showed high diversity, consisting mostly of singleton and doubleton phylotypes. The majority of the phylotypes were typical lake bacteria including some potential pathogens and toxin metabolising bacteria, suggesting that the dominant toxic cyanobacteria did not have any significant effect on the bacterial community structure. In the sediment, 2 years after the water bloom, no bloom-forming cyanobacteria were retrieved, suggesting that they cannot be preserved in the sediment. Similar to the water column, sediment bacterial diversity was also high, consisting mostly of yet-uncultured bacteria that are related to environments where organic matter degradation takes place.