Growth of Streptomyces hygroscopicus in rotating-wall bioreactor under simulated microgravity inhibits rapamycin production
Growth of Streptomyces hygroscopicus in rotating-wall bioreactor under simulated microgravity inhibits rapamycin production
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DOI：
10.1007/s002539900303
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发表时间：
2000-07-01
影响因子：
5
通讯作者：
Demain, AL
中科院分区：
文献类型：
--
作者：
Fang, A;Pierson, DL;Demain, AL
吸湿链霉菌在模拟微重力条件下在旋转壁生物反应器中生长，导致颗粒状生长，降低干细胞重量，抑制雷帕霉素的产生。在生物反应器中加入聚四氟乙烯珠；生长变得更少颗粒，干细胞重量增加，但雷帕霉素的产生仍然明显受到抑制。与正常重力条件下观察到的细胞结合雷帕霉素的更高百分比相比，模拟微重力条件下的生长有利于雷帕霉素的细胞外生产。
Growth of Streptomyces hygroscopicus under conditions of simulated microgravity in a rotating-wall bioreactor resulted in a pellet form of growth, lowered dry cell weight, and inhibition of rapamycin production. With the addition of Teflon beads to the bioreactor; growth became much less pelleted, dry cell weight increased but rapamycin production was still markedly inhibited. Growth under simulated microgravity favored extracellular production of rapamycin, in contrast to a greater percentage of cell-bound rapamycin observed under normal gravity conditions.