Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.
Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.
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DOI：
10.2147/ott.s61794
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发表时间：
2014
影响因子：
4
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Feng Z;Hao W;Lin X;Fan D;Zhou J
确定四柱黄酮类化合物的抗肿瘤活性及其对小鼠调节性 T 细胞 (Treg) 的抑制作用。从 Tetrastigma hemsleyanum Diels et Gilg 的根（Tetrastigma hemsleyanum Diels et Gilg）中分离出总黄酮，并在接种 Lewis 肺癌 (LLC) 细胞后通过口服管饲法给予 C57BL/6 小鼠。检查了总黄酮对体内肿瘤生长的影响。采用流式细胞术研究对Tregs的影响，采用酶联免疫吸附法分析肿瘤接种和黄酮类药物给药后血清转化生长因子β、前列腺素E2、环氧合酶2水平的变化。来自 T. hemsleyanum Diels et Gilg 的总黄酮显着抑制接种 LLC 的 C57BL/6 小鼠的肿瘤生长。这些黄酮类化合物可显着抑制荷瘤小鼠中调节性 T 细胞的发育。进一步的研究表明，总黄酮显着降低荷瘤小鼠血清中转化生长因子β、前列腺素E2和环氧合酶2的水平，这可能是抑制Tregs的原因。 T. hemsleyanum Diels et Gilg 总黄酮的抗肿瘤活性与小鼠肿瘤模型中 Tregs 的抑制有关。来自 T. hemsleyanum Diels et Gilg 的总黄酮类化合物可用作癌症预防和治疗中的抗肿瘤剂。
To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice. Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration. Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs. The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.