INCREASED EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH PERMEABILITY FACTOR IN THE RAT OVARY FOLLOWING AN OVULATORY GONADOTROPIN STIMULUS - POTENTIAL ROLES IN FOLLICLE RUPTURE
INCREASED EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH PERMEABILITY FACTOR IN THE RAT OVARY FOLLOWING AN OVULATORY GONADOTROPIN STIMULUS - POTENTIAL ROLES IN FOLLICLE RUPTURE
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DOI：
10.1095/biolreprod52.6.1426
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发表时间：
1995-06-01
影响因子：
3.6
通讯作者：
KOOS, RD
中科院分区：
文献类型：
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作者：
KOOS, RD
排卵伴随着卵泡周围毛细血管通透性的大幅度增加，在排卵刺激后几小时开始。由此产生的水肿可能在排卵以及CL的形成和血管化中起作用。血管内皮生长/通透性因子（VEG/PF）是一种内皮细胞的特异性有丝分裂原，同时也是一种强有力的血管通透性刺激因子。本研究的目的是探讨排卵刺激是否能诱导卵巢VEG/PF的表达增加。雌性大鼠在27日龄时用8 IU eCG致敏。促排卵后第二天下午注射hCG（5 IU）诱导卵泡成熟和排卵。在0、1、2、4、8、10和18 h后取出卵巢。采用RT-PCR方法检测VEG/PF mRNA水平。在hCG后1 - 4 h，整个卵巢中VEG/PF 120和VEG/PF 164转录物的稳态水平显著增加（约8倍）。颗粒细胞和卵泡膜/间质组织中均检测到增加。在10和18 h（第1天CL）维持高水平表达。因此，排卵前卵泡血管通透性的增加与VEG/PF表达的显著、持续增加密切相关。因此，VEG/PF可能在这种增加中起重要作用，因此，在排卵过程中以及随后的CL血管化中起重要作用。
Ovulation is accompanied by a large increase in the permeability of the capillaries surrounding the follicle, beginning a few hours after the ovulatory stimulus. The resulting edema may play a role in ovulation as well as in the formation and vascularization of the CL. Vascular endothelial growth/permeability factor (VEG/PF) is both a specific mitogen for endothelial cells and a potent stimulator of vascular permeability, The purpose of this study was to determine whether or not the ovulatory stimulus induces an increase in the expression of VEG/PF in the ovary. Female rats were primed with 8 IU of eCG at 27 days of age. Follicular maturation and ovulation were induced with an injection of hCG (5 IU) in the early afternoon on the second day after priming. Ovaries were removed 0, 1, 2, 4, 8, 10, and 18 h later. VEG/PF mRNA levels were compared through use of quantitative reverse transcription-polymerase chain reaction (RT-PCR). There was a marked increase (approximately 8 fold) in steady state levels of the transcripts for VEG/PF120 and VEG/PF164 between 1 and 4 h after hCG in whole ovaries. Increases were detectable both in granulosa cells and in thecal/stromal tissue. The high level of expression was maintained at 10 and 18 h (Day 1 CL). Thus, the preovulatory increase in follicular vascular permeability is closely associated with a marked, sustained increase in VEG/PF expression. VEG/PF, therefore, may play an important role in that increase and, consequently, in the process of ovulation as well as the subsequent vascularization of the CL.