Typing of herpes simplex virus with type-specific human immunoglobulin M in an indirect immunofluorescence assay
Typing of herpes simplex virus with type-specific human immunoglobulin M in an indirect immunofluorescence assay
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在间接免疫荧光测定中使用特异性人免疫球蛋白 M 对单纯疱疹病毒进行分型
DOI：
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发表时间：
1982
影响因子：
9.4
通讯作者：
F. Chow
中科院分区：
文献类型：
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作者：
S. Spruance;C. Golden;E. Kern;M. Katz;F. Chow
对9名疑似原发性单纯疱疹病毒1型（HSV-1）或2型（HSV-2）感染者的血清进行筛选，以鉴定含有HSV型特异性免疫球蛋白M（IgM）的血清。然后将选择的血清用于IgM特异性间接免疫荧光抗体HSV分型测定（专利申请中）。为了评估该程序，29株HSV分离株在连续培养的人羊膜细胞中生长，固定，并用作间接免疫荧光的底物。病毒类型的确定基于用荧光素缀合的抗人IgM染色后用HSV-1和HSV-2特异性抗血清的底物的荧光强度。用限制性内切酶对29株分离株进行分型，其中HSV-2型18株，HSV-1型11株。IgM特异性间接免疫荧光抗体测定的结果是相同的限制性内切酶消化的27个分离株; 2个分离株未能充分复制在测试细胞。IgM特异性间接免疫荧光抗体法是一种简便、快速、准确的方法，可用于临床病毒学实验室。
Sera from nine individuals with suspected primary herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2) infection were screened to identify those containing HSV type-specific immunoglobulin M (IgM). Selected sera were then utilized in an IgM-specific indirect immunofluorescent-antibody HSV-typing assay (patent pending). To evaluate the procedure, 29 HSV isolates were grown in cultures of continuous human amnion cells, fixed, and used as substrates for indirect immunofluorescence. Determination of virus type was based on intensity of fluorescence of the substrate with HSV-1- and HSV-2-specific antisera after staining with fluorescein-conjugated anti-human IgM. Typing of the isolates by restriction endonuclease digestion showed that of 29, 18 were HSV-2 and 11 were HSV-1. Results by IgM-specific indirect immunofluorescent-antibody assay were identical to those by restriction endonuclease digestion for 27 of the isolates; 2 isolates failed to replicate adequately in the test cells. The IgM-specific indirect immunofluorescent-antibody procedure appears to be a simple, rapid, and accurate technique which could be of use to clinical virology laboratories.