Expression of MDC/CCL22 and its receptor CCR4 in rheumatoid arthritis, psoriatic arthritis and osteoarthritis
Expression of MDC/CCL22 and its receptor CCR4 in rheumatoid arthritis, psoriatic arthritis and osteoarthritis
复制标题
DOI：
10.1016/j.cyto.2009.10.005
复制
发表时间：
2010-01-01
期刊：
影响因子：
3.8
通讯作者：
Deleuran, Bent
中科院分区：
文献类型：
--
作者：
Flytlie, Helene Aarslev;Hvid, Malene;Deleuran, Bent
类风湿性关节炎（RA）和银屑病关节炎（PsA）的发病机制涉及趋化因子调节异常。趋化因子受体 CCR4 对于 T 细胞迁移到皮肤是必需的。因此，我们通过检查 RA、PsA 和骨关节炎 (OA) 患者，研究了 CCR4 及其配体巨噬细胞衍生趋化因子 (MDC/CCL22) 是否可以参与皮肤和关节之间疾病的传播。在 RA 和 PsA 患者的滑液中，我们观察到与 OA 患者相比，MDC/CCL22 水平显着升高。此外，与 OA 和健康志愿者相比，RA 和 PsA 血浆中的 MDC/CCL22 蛋白升高。流式细胞术显示大多数CD4(+)CCR4(+)淋巴细胞也共表达CD45RO。 MDC/CCL22 水平和 CCR4 表达均与 CRP 无关。 RA 和 OA 滑膜的免疫组织化学显示单核细胞和内皮细胞表达 CCR4。我们的结果表明，MDC/CCL22 存在于 RA 和 OA 患者的滑膜内，并且在 RA 和 PsA 患者的滑液中含量很高。这将使表达 CCR4 的记忆细胞迁移，支持 MDC/CCR4 在吸引皮肤特异性记忆 T 细胞到关节方面发挥作用。 (C) 2009 Elsevier Ltd. 保留所有权利。
The pathogenesis of rheumatoid arthritis (RA) and psoriatic arthritis (PsA) involves an abnormal chemokine regulation. The chemokine receptor CCR4 is necessary for T cell migration to the skin. We, therefore, studied if CCR4 and its ligand macrophage-derived chemokine (MDC/CCL22) could participate in spreading the disease between skin and joints by examining RA, PsA and osteoarthritis (OA) patients. In synovial fluid from RA and PsA patients we observed a significantly higher MDC/CCL22 level compared to OA patients. Additionally, the MDC/CCL22 protein was found to be elevated in RA and PsA plasma compared to OA and healthy volunteers. Flow cytometry revealed that most CD4(+)CCR4(+) lymphocytes also co-expressed CD45RO. Neither the MDC/CCL22 level nor the expression of CCR4 correlated to CRP. Immunohistochemistry of the RA and OA synovial membrane demonstrated CCR4 to be expressed by mononuclear cells and endothelial cells. Our results show that MDC/CCL22 is present within the synovial membrane of RA and OA patients and in high amount in the synovial fluid of patients with RA and PsA. This will enable migration of CCR4 expressing memory cells supporting that MDC/CCR4 could play a role in attracting skin specific memory T cells to the joints. (C) 2009 Elsevier Ltd. All rights reserved.