Effects of Scalp Electroacupuncture on the Pi3K/Akt Signalling Pathway and Apoptosis of Hippocampal Neurons in a Rat Model of Cerebral Palsy
Effects of Scalp Electroacupuncture on the Pi3K/Akt Signalling Pathway and Apoptosis of Hippocampal Neurons in a Rat Model of Cerebral Palsy
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DOI：
10.1136/acupmed-2016-011335
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发表时间：
2018-04
影响因子：
2.5
通讯作者：
Hanhong Zhang;Jing Gao;Mengmeng Wang;Xuefeng Yu;Xiuhua Lv;H. Deng;Xiangwei Fan;Kaiyun Chen
中科院分区：
文献类型：
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作者：
Hanhong Zhang;Jing Gao;Mengmeng Wang;Xuefeng Yu;Xiuhua Lv;H. Deng;Xiangwei Fan;Kaiyun Chen
背景来自临床报道的大量证据表明，大多数脑性瘫痪(CP)患者受益于全面的康复运动训练计划。当在与运动区投影相对应的位置应用头皮电针(EA)与康复运动训练相结合时，这些进步得到加强。然而，关于这些影响的机制基础的信息很少。目的探讨电针刺激运动区头皮投射部位是否通过调节PI3K/Akt信号通路抑制慢性阻塞性肺疾病大鼠海马神经元的凋亡。方法雄性SD大鼠50只，建立脑性瘫痪手术模型。将其余45只大鼠随机分为未治疗组(CP组，n=15)、单纯电针治疗组(CP+EA组，n=15)和电针联合PI3K/Akt抑制剂治疗组(CP+EA+LY294002组，n=15)。另设健康阴性对照组进行假手术(对照组，n=15)。在CP+电针组和CP+电针+LY294002组中，在运动区投影对应的位置对头皮表面进行电针。分别于CP诱导后7、14、21d检测BBB、BBB运动评分、Akt和p-Akt蛋白表达(Western印迹法)和海马神经细胞凋亡率(组织病理学)。结果与未治疗的CP大鼠相比，接受头皮电针治疗的CP大鼠在各个时间点的行为学评分均有改善，海马神经细胞凋亡率减少，Akt和p-Akt的表达水平升高(p<；0.05)。CP+EA+LY294002组与未治疗组比较，差异无统计学意义。结论头皮电针对PI3K/Akt信号通路的影响可能是电针抑制大鼠海马神经细胞凋亡、改善慢性阻塞性肺疾病相关缺陷的机制之一。
Background Substantial evidence from clinical reports has established that most cerebral palsy (CP) patients benefit from a comprehensive rehabilitation exercise training programme. Such advances are enhanced when scalp electroacupuncture (EA), applied at a location corresponding to the projection of the motor area, is combined with rehabilitation exercise training. However, little information exists regarding the mechanistic basis for these effects. Objective To examine whether EA stimulation within the scalp projection location of the motor area can inhibit apoptosis of hippocampal neurons by regulating the PI3k/Akt signalling pathway in a rat model of CP. Methods Fifty male Sprague-Dawley rats underwent surgical modelling of CP. Five were used to confirm successful establishment of the model and the remaining 45 rats were randomly divided into one of three groups that remained untreated (CP group, n=15) or received EA treatment alone (CP+EA group, n=15) or EA in combination with a PI3K/Akt inhibitor (CP+EA+LY294002 group, n=15)). An otherwise healthy negative control group of rats undergoing sham surgery was also included (Control group, n=15). In the CP+EA and CP+EA+LY294002 groups, EA was applied to the scalp surface at alocation corresponding to the projection of the motor area. Basso, Beattie and Bresnahan (BBB) locomotor scores, hippocampal protein expression of Akt and p-Akt (by Western blot analysis) and neuronal apoptosis in hippocampal tissue (by histopathology) were assessed at 7, 14 and 21 days post-CP induction. Results CP rats receiving scalp EA treatment demonstrated improved behavioural scores, less hippocampal neuronal apoptosis and higher expression levels of Akt and p-Akt (p<0.05) at all time points studied compared with untreated CP rats. There were no significant differences observed between CP+EA+LY294002 and untreated CP model groups. Conclusions The effects of scalp EA on the PI3K/ Akt signalling pathway may represent one of the mechanisms involved in the inhibition of hippocampal neuronal apoptosis and improvement of deficits associated with CP in a rat model.