Mechanism of Virus Inactivation by Cold Atmospheric-Pressure Plasma and Plasma-Activated Water.
Mechanism of Virus Inactivation by Cold Atmospheric-Pressure Plasma and Plasma-Activated Water.
复制标题
冷常压等离子体和等离子体活化水灭活病毒的机制
DOI：
10.1128/aem.00726-18
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发表时间：
2018-09-01
影响因子：
4.4
通讯作者：
Kong MG
中科院分区：
文献类型：
--
作者：
Guo L;Xu R;Gou L;Liu Z;Zhao Y;Liu D;Zhang L;Chen H;Kong MG
病原性和传染性病毒的污染严重威胁着人类健康和畜牧业。目前的消毒方法具有不同的缺点，例如不方便和消毒副产物的污染（例如，氯消毒）。在这项研究中，发现氩气中的大气表面等离子体与空气和等离子体活化水混合可以有效地杀灭噬菌体，并且等离子体活化水在长时间储存后仍然具有很强的抗病毒活性。此外，研究表明噬菌体失活与单线态氧对核酸和蛋白质的损伤有关。了解基于等离子体的治疗的生物学效应有助于将等离子体开发成一种方便且无副产物的新型消毒策略。摘要病毒引起严重的病原污染，严重影响环境和人类健康。冷大气压等离子体可有效灭活病原菌;然而，等离子体灭活病毒的机制尚不完全清楚。在这项研究中，表面等离子体在氩气与1%的空气和等离子体活化水混合，用于处理含有噬菌体的水。两种试剂均以时间依赖性方式有效灭活噬菌体T4、Φ174和MS 2。长期储存对血浆活化水的抗病毒活性有边际影响。DNA和蛋白质分析显示，由等离子体产生的反应性物质破坏核酸和蛋白质，与形态学检查一致，表明等离子体处理引起噬菌体的聚集。单线态氧清除剂可减轻噬菌体的失活，表明单线态氧在此过程中起主要作用。我们的研究结果提供了一个潜在的有效的消毒策略，以打击环境中的病毒，使用冷大气压等离子体和等离子体活化水。病原性和传染性病毒的污染严重威胁人类健康和畜牧业。目前的消毒方法具有不同的缺点，例如不方便和消毒副产物的污染（例如，氯消毒）。在这项研究中，发现氩气中的大气表面等离子体与空气和等离子体活化水混合可以有效地杀灭噬菌体，并且等离子体活化水在长时间储存后仍然具有很强的抗病毒活性。此外，研究表明噬菌体失活与单线态氧对核酸和蛋白质的损伤有关。了解基于等离子体的治疗的生物学效应有助于将等离子体开发成一种方便且无副产物的新型消毒策略。
Contamination with pathogenic and infectious viruses severely threatens human health and animal husbandry. Current methods for disinfection have different disadvantages, such as inconvenience and contamination of disinfection by-products (e.g., chlorine disinfection). In this study, atmospheric surface plasma in argon mixed with air and plasma-activated water was found to efficiently inactivate bacteriophages, and plasma-activated water still had strong antiviral activity after prolonged storage. Furthermore, it was shown that bacteriophage inactivation was associated with damage to nucleic acids and proteins by singlet oxygen. An understanding of the biological effects of plasma-based treatment is useful to inform the development of plasma into a novel disinfecting strategy with convenience and no by-product. ABSTRACT Viruses cause serious pathogenic contamination that severely affects the environment and human health. Cold atmospheric-pressure plasma efficiently inactivates pathogenic bacteria; however, the mechanism of virus inactivation by plasma is not fully understood. In this study, surface plasma in argon mixed with 1% air and plasma-activated water was used to treat water containing bacteriophages. Both agents efficiently inactivated bacteriophages T4, Φ174, and MS2 in a time-dependent manner. Prolonged storage had marginal effects on the antiviral activity of plasma-activated water. DNA and protein analysis revealed that the reactive species generated by plasma damaged both nucleic acids and proteins, consistent with the morphological examination showing that plasma treatment caused the aggregation of bacteriophages. The inactivation of bacteriophages was alleviated by the singlet oxygen scavengers, demonstrating that singlet oxygen played a primary role in this process. Our findings provide a potentially effective disinfecting strategy to combat the environmental viruses using cold atmospheric-pressure plasma and plasma-activated water. IMPORTANCE Contamination with pathogenic and infectious viruses severely threatens human health and animal husbandry. Current methods for disinfection have different disadvantages, such as inconvenience and contamination of disinfection by-products (e.g., chlorine disinfection). In this study, atmospheric surface plasma in argon mixed with air and plasma-activated water was found to efficiently inactivate bacteriophages, and plasma-activated water still had strong antiviral activity after prolonged storage. Furthermore, it was shown that bacteriophage inactivation was associated with damage to nucleic acids and proteins by singlet oxygen. An understanding of the biological effects of plasma-based treatment is useful to inform the development of plasma into a novel disinfecting strategy with convenience and no by-product.