Establishment of novel technical methods for evaluating brain edema and lesion volume in stroked rats: A standardization of measurement procedures
Establishment of novel technical methods for evaluating brain edema and lesion volume in stroked rats: A standardization of measurement procedures
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DOI：
10.1016/j.brainres.2019.04.022
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发表时间：
2019-09-01
期刊：
影响因子：
2.9
通讯作者：
Zlotnik, Alexander
中科院分区：
文献类型：
--
作者：
Boyko, Matthew;Zvenigorodsky, Vladislav;Zlotnik, Alexander
中风在高发病率和高死亡率中起着重要作用。破译其机制和病理生理学对于创造新的药物和疗法至关重要。以往的大多数中风动物模型，旨在确定中风后脑损伤的程度和部位，都需要动物牺牲，这除了伦理上的考虑外，也否定了对同一只大鼠进行后续研究的能力。由于这些失败，临床磁共振扫描仪用于评估小动物模型的使用一直在增加。特别用于小口径动物的磁共振成像扫描仪可用于啮齿动物大脑的高分辨率磁共振成像。然而，高成本和稀缺性严重影响了这些扫描仪的可用性。在我们的研究中，我们试图建立一个统一的磁共振成像方案来评估大鼠中风。我们使用了一台3特斯拉磁共振成像临床扫描仪，以及另一台临床设备，目的是增加其重复性。研究结果证实了一种新的磁共振成像方案，将磁共振成像测量的梗死区与组织学检查的“金标准”进行了比较。我们在一台3特斯拉磁共振成像临床扫描仪上使用传统的八通道仅接收线圈进行了实验程序。两种方法在数量和质量上都有显著的相关性。最后，我们证明了临床磁共振成像扫描仪是一种高精度和敏感的图像分析仪器，用于评估脑卒中实验大鼠模型的梗死区和脑水肿。
Stroke plays a role in high morbidity and mortality. Deciphering its mechanisms and pathophysiology is critical for the creation of new drugs and therapies. Most of the previous animal models of stroke, aimed at identifying the extent and location of brain injury following stroke, require animal sacrifice, which, besides ethical considerations, also negates the ability for follow up studies with the same rats. Because of these failures, the use of clinical magnetic resonance scanners for evaluating small animal models has been increasing. Magnetic resonance imaging scanners used particularly for small-bore animals are eligible for use in high-resolution magnetic resonance imaging of rodent brains. However, high costs and scarcity factor heavily in the rare availability of these scanners.In our investigation, we sought to establish a unitary magnetic resonance imaging protocol for stroke assessment in rats. We made use of a 3-Tesla magnetic resonance imaging clinical scanner, as well as another clinical equipment, with the purpose of increasing its reproducibility. The results of inquest validated a new magnetic resonance imaging protocol, comparing a magnetic resonance imaging-measured infarcted zone to the "gold standard" of histological examination. We carried out the experimental procedure on a 3 Tesla magnetic resonance imaging clinical scanner using a conventional eight-channel receive-only coil.The two methods produced remarkable quantitative and qualitative correlations between them. Conclusively, we showed the clinical magnetic resonance imaging scanner to be a high-precision and sensitive image analysis instrument for evaluating both the infarct zone and the brain edema in a stroke experimental rat model.