A novel method for determining linkage between DNA sequences: hybridization to paired probe arrays
A novel method for determining linkage between DNA sequences: hybridization to paired probe arrays
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DOI：
10.1093/nar/27.6.1485
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发表时间：
1999-03-15
影响因子：
14.9
通讯作者：
Chee, M
中科院分区：
文献类型：
--
作者：
Gentalen, E;Chee, M
合作杂交已用于建立DNA链上两个基因座之间的物理连锁。通过与新型高密度寡核苷酸阵列杂交来检测连锁。阵列上的每个合成位置含有两种不同探针序列的混合物。两种探针中的每一种都可以独立地与不同的靶序列杂交，但是如果两种靶序列物理连接，则杂交产率协同增加。产生和控制非线性效应的能力为寡核苷酸阵列杂交的应用提出了许多可能性，该方法已被用于分配连锁在50：50的DNA混合物含有单核苷酸多态性（SNP）分开17，693，1350和2038 bp，并重建单倍型，其他潜在的用途包括增加杂交的特异性在突变检测和基因表达监测应用，确定SNP单倍型，表征重复序列，如短串联重复序列，并帮助重叠群组装在测序杂交。
Cooperative hybridization has been used to establish physical linkage between two loci on a DNA strand. Linkage was detected by hybridization to a new type of high-density oligonucleotide array. Each synthesis location on the array contains a mixture of two different probe sequences, Each of the two probes can hybridize independently to a different target sequence, but if the two target sequences are physically linked there is a cooperative increase in hybridization yield, The ability to create and control non-linear effects raises a host of possibilities for applications of oligonucleotide array hybridization, The method has been used to assign linkage in 50:50 mixtures of DNA containing single nucleotide polymorphisms (SNPs) separated by 17, 693, 1350 and 2038 bp and to reconstruct haplotypes, Other potential uses include increasing the specificity of hybridization in mutation detection and gene expression monitoring applications, determining SNP haplotypes, characterizing repetitive sequences, such as short tandem repeats, and aiding contig assembly in sequencing by hybridization.