Gene Expression Response to Stony Coral Tissue Loss Disease Transmission in M. cavernosa and O. faveolata From Florida
Gene Expression Response to Stony Coral Tissue Loss Disease Transmission in M. cavernosa and O. faveolata From Florida
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DOI：
10.3389/fmars.2021.681563
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发表时间：
2021-06
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通讯作者：
N. Traylor-Knowles;Michael T Connelly;Benjamin D Young;Katherine Eaton;E. Muller;V. Paul;Blake Ushijima;Allyson DeMerlis;Melissa K. Drown;A. Goncalves;N. Kron;G. Snyder;Cecily Martin;Kevin Rodriguez
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作者：
N. Traylor-Knowles;Michael T Connelly;Benjamin D Young;Katherine Eaton;E. Muller;V. Paul;Blake Ushijima;Allyson DeMerlis;Melissa K. Drown;A. Goncalves;N. Kron;G. Snyder;Cecily Martin;Kevin Rodriguez
自2014年以来，由于石珊瑚组织损失病（SCTLD），佛罗里达州珊瑚礁内的珊瑚以前所未有的速度死亡。在这里，我们描述了2019年至2020年间在史密森海洋站和莫特海洋实验室进行的三种不同的SCTLD传播实验的转录组学结果，这些实验是在珊瑚Orbicella faveolata和Montastraea海龙珊瑚上进行的。总体而言，与健康菌落相比，患病的黄豆豆有2194个差异表达基因（DEGs），而患病的海绵状乳杆菌与健康菌落相比有582个差异表达基因（DEGs）。许多重要的deg与免疫、细胞外基质重排和细胞凋亡有关。这些包括但不限于过氧化物酶、胶原蛋白、bax样蛋白、纤维蛋白原样蛋白、酪氨酸激酶和转化生长因子β。发现了一个与疾病传播显著相关的基因模块。该模块具有许多凋亡和免疫基因，模块成员数高，表明珊瑚在SCTLD传播过程中发生了复杂的凋亡和免疫反应。总的来说，我们发现黄豆瓣和海绵状芽胞杆菌对SCTLD表现出免疫、细胞凋亡和组织重排反应。我们建议未来的研究应该集中在检查感染的早期时间点，在病变出现之前，以了解SCTLD的激活机制。
Since 2014, corals within Florida’s Coral Reef have been dying at an unprecedented rate due to stony coral tissue loss disease (SCTLD). Here we describe the transcriptomic outcomes of three different SCTLD transmission experiments performed at the Smithsonian Marine Station and Mote Marine Laboratory between 2019 and 2020 on the corals Orbicella faveolata and Montastraea cavernosa. Overall, diseased O. faveolata had 2194 differentially expressed genes (DEGs) compared with healthy colonies, whereas diseased M. cavernosa had 582 DEGs compared with healthy colonies. Many significant DEGs were implicated in immunity, extracellular matrix rearrangement, and apoptosis. These included, but not limited to, peroxidases, collagens, Bax-like, fibrinogen-like, protein tyrosine kinase, and transforming growth factor beta. A gene module was identified that was significantly correlated to disease transmission. This module possessed many apoptosis and immune genes with high module membership indicating that a complex apoptosis and immune response is occurring in corals during SCTLD transmission. Overall, we found that O. faveolata and M. cavernosa exhibit an immune, apoptosis, and tissue rearrangement response to SCTLD. We propose that future studies should focus on examining early time points of infection, before the presence of lesions, to understand the activating mechanisms involved in SCTLD.