Biomechanical analysis of the Rolled (Rld) leaf phenotype of maize
Biomechanical analysis of the Rolled (Rld) leaf phenotype of maize
复制标题
DOI：
10.2307/2656848
复制
发表时间：
2000-05-01
影响因子：
3
通讯作者：
Silk, WK
中科院分区：
文献类型：
--
作者：
Hay, JO;Moulia, B;Silk, WK
玉米（Zea mays） Rldl-O/t突变对叶片表型的多效性影响包括抑制正常的横向展开，上下表皮极性颠倒，纵向形状明显变直。根据工程壳理论，叶片纵、横习惯之间可能存在力学耦合，即叶片下垂本身可能产生纵向矫直。我们用定量曲率测量和机械解耦实验来检验这种可能性。在Rld-O/+突变体和同胞+/+分离群体中，研究了叶片在自重作用下的弹性弯曲、机械耦合和静止状态对叶片纵向和横向习性的贡献。弹性弯曲和曲率耦合是相对不重要的。Rld-O/+突变不仅经历了展开过程，而且还经历了生长叶片的发育纵向弯曲，导致中脉更直，叶片卷曲。因此，Rldl-O/+突变体是研究玉米叶片组织极性与差异曲率发育之间关系的合适模型。由于+/+型比Rldl-O/+型在叶片背面有更丰富的厚壁组织，因此硬化的梯度可能有助于中脉曲率的发展。
The pleiotropic effects of the Rldl-O/t mutation of Zea mays (Poaceae) on leaf phenotype include a suppression of normal transverse unrolling, a reversed top/bottom epidermal polarity, and an apparently straighter longitudinal shape. According to engineering shell theory, there might be mechanical coupling between transverse and longitudinal habit, i.e., the leaf lolling itself might produce the longitudinal straightening. We tested this possibility with quantitative curvature measurements and mechanical uncoupling experiments. The contributions of elastic bending under self weight, mechanical coupling, and rest state of leaf parts to the longitudinal and transverse habit were assessed in Rld-O/+ mutants and a population of sibling +/+ segregants. Elastic bending and curvature coupling are shown to be relatively unimportant. The Rld-O/+ mutation is shown to after not only the unrolling process, but also the developmental longitudinal curving in the growing leaf, leading to a straighter midrib and a rolled lamina. The Rldl-O/+ mutant is thus a suitable model to study the relation between tissue polarity and differential curvature development in the maize leaf. Since on the abaxial side of the leaf more abundant sclerenchyma is found in +/+ than in Rldl-O/+, a gradient in sclerification may contribute to the development of midrib curvature.