BIOCHEMICAL SUBTYPES OF OLIGODENDROCYTE IN THE ANTERIOR MEDULLARY VELUM OF THE RAT AS REVEALED BY THE MONOCLONAL-ANTIBODY RIP
BIOCHEMICAL SUBTYPES OF OLIGODENDROCYTE IN THE ANTERIOR MEDULLARY VELUM OF THE RAT AS REVEALED BY THE MONOCLONAL-ANTIBODY RIP
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DOI：
10.1002/glia.440140304
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发表时间：
1995-07-01
期刊：
影响因子：
6.2
通讯作者：
BERRY, M
中科院分区：
文献类型：
--
作者：
BUTT, AM;IBRAHIM, M;BERRY, M
用功能未知的少突胶质细胞标记物Rip的单抗对大鼠前髓鞘内的少突胶质细胞进行了研究。RIP和CAII双免疫荧光标记的共聚焦显微镜成像显示，少突胶质细胞有两种不同的生化和形态特征：RIP+CAII+或RIP+CAII-。RIP和髓鞘碱性蛋白(MBP)或胶质纤维酸性蛋白(GFAP)的双重免疫荧光标记提供了直接证据，表明RIP标记的细胞是表型少突胶质细胞，并证实RIP不识别星形胶质细胞。对于小直径轴突，RIP+CAII+少突胶质细胞支持大量的髓鞘，而对于大直径轴突，RIP+CAII-少突胶质细胞支持较少的髓鞘。在形态上，RIP+CAII+少突胶质细胞对应于经典命名的I型或II型，而RIP+CAII-少突胶质细胞对应于III型和IV型。结果表明，小直径纤维髓鞘的少突胶质细胞与大直径纤维的少突胶质细胞存在生化差异。(C)1995年Wiley-Liss公司
Oligodendrocytes were studied in the anterior medullary velum (AMV) of the rat using the monoclonal antibody Rip, an oligodendrocyte marker of unknown function. Confocal microscopic imaging of double immunofluorescent labelling with antibodies to Rip and carbonic anhydrase II (CAII) revealed two biochemically and morphologically distinct populations of oligodendrocyte which were either Rip+CAII+ or Rip+CAII-. Double immunofluorescent labelling with Rip and myelin basic protein (MBP) or glial fibrillary acidic protein (GFAP) provided direct evidence that Rip-labelled cells were phenotypically oligodendrocytes and confirmed that Rip did not recognise astrocytes. Oligodendrocytes which were Rip+ CAII+ supported numerous myelin sheaths for small diameter axons, whilst Rip+CAII- oligodendrocytes supported fewer myelin sheaths for large diameter axons. Morphologically, Rip+ CAII+ oligodendrocytes corresponded to types I or II of classical nomenclature, whilst Rip+CAII- oligodendrocytes corresponded to types III and IV. The results demonstrated a biochemical difference between oligodendrocytes which myelinated small and large diameter fibres. (C) 1995 Wiley-Liss, Inc.