Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India.
Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India.
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DOI：
10.1002/jmv.20862
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发表时间：
2007-05
影响因子：
12.7
通讯作者：
中科院分区：
文献类型：
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作者：
儿童胃肠炎是全世界儿童发病率和死亡率的主要原因，特别是在发展中国家。人们越来越认识到，人类杯状病毒(HuCV)包括诺如病毒(NOV)和沙门氏菌病毒(SAV)，在暴发和非暴发环境中都很重要。这项研究旨在确定在印度住院儿童和社区儿童粪便中检测到的杯状病毒的特征。这项研究检查了350份来自到医院就诊的急性胃肠炎儿童的粪便样本，673份来自社区儿童的样本，500份来自腹泻儿童的样本，以及173份来自非腹泻儿童的样本。通过逆转录-聚合酶链式反应(RT-PCR)和编码依赖于RNA的RNA聚合酶(RdRp)和/或跨越开放阅读框架(ORF)1和2(ORF1/ORF2)连接的区域的部分序列对菌株进行鉴定。350份住院患儿标本中有68份(19.4%)阳性，其中沙门氏菌和新城疫分别占感染总数的5.1%和15.1%。HuCVs与其他肠道病原体混合感染占受检儿童总数的9.4%。673份社区儿童标本杯状病毒阳性68份，占10.1%，其中沙门氏菌和新城疫分别占3.4和6.6%。社区队列中有症状和无症状儿童分别占55/500(11%)和13/173(7.5%)，SAV占17/500(3.4%)，NOV占38/500(7.6%)。这是印度首次在医院和社区对流行性杯状病毒进行基因分型，并增加了这些病毒在印度儿童胃肠炎中的作用的证据。
Pediatric gastroenteritis is a major cause of childhood morbidity and mortality worldwide, especially in developing countries. It has been increasingly recognised that human caliciviruses (HuCV), comprising noroviruses (NoV), and sapoviruses (SaV), are important in both outbreak and non-outbreak settings. This study aimed to characterise caliciviruses detected in the faeces of hospitalized children and children in the community in India. This study examined 350 faecal samples from children presenting to the hospital with acute gastroenteritis and 673 samples collected from children in the community, 500 from children with diarrhea, and 173 samples from children without diarrhea. Strain characterisation was performed by reverse transcription-polymerase chain reaction (RT-PCR) and partial sequencing of the gene encoding the RNA-dependent RNA polymerase (RdRp) and/or a region spanning the open reading frames (ORFs) 1 and 2 (ORF1/ORF2) junction. A total of 68 of 350 specimens (19.4%) from hospitalized children were positive, and SaV and NoV accounted for 5.1 and 15.1% of the infections, respectively. Mixed infections of HuCVs with other enteric pathogens were seen in 9.4% of the total children tested. Sixty-eight out of 673 (10.1%) samples collected from children in the community were positive for caliciviruses, and SaV and NoV accounted for 3.4 and 6.6% of the infections. In the community cohort 55/500 (11%) and 13/173 (7.5%) were from symptomatic and asymptomatic children, respectively, and SaVs accounted for 17/500 (3.4%) and NoVs for 38/500 (7.6%) of the symptomatic infections. This is the first report of genotyping of circulating caliciviruses in both hospital and community in India and has increased the evidence for the role of these viruses in pediatric gastroenteritis in India.