Viscosity of bovine serum albumin aqueous solutions as a function of temperature and concentration
Viscosity of bovine serum albumin aqueous solutions as a function of temperature and concentration
复制标题
DOI：
10.1016/0141-8130(95)01057-2
复制
发表时间：
1996-02-01
影响因子：
8.2
通讯作者：
Monkos, K
中科院分区：
文献类型：
--
作者：
Monkos, K
本文介绍了在 5°C 至 45°C 温度范围内对牛血清白蛋白 (BSA) 水溶液进行粘度测定的结果。在这些测量的基础上，建立了将相对粘度 eta(r) 与温度 T 和溶解蛋白浓度 c 连接起来的通用公式：eta(r) = exp[c/α-β c(-(B) over tilde + (D) over tilde T + Delta (E) over波形符/RT)]波形符上的α、β、波形符上的(B)、波形符上的(D) 和波形符上的Delta (E) 量在下面的文本中描述。还给出了该公式的简单证明。该关系立即给出门尼近似，并允许在该近似中预测参数 S 和自拥挤因子 K 的值。通过应用该公式的渐近形式，可以计算特性粘度和哈金斯系数等流变量。
This paper presents the results of viscosity determinations on aqueous solutions of bovine serum albumin (BSA) at a wide range of concentrations and at temperatures ranging from 5 degrees C to 45 degrees C. On the basis of these measurements a general formula connecting the relative viscosity eta(r) with temperature T and concentration c of the dissolved proteins was established:eta(r) = exp[c/alpha-beta c(-(B) over tilde + (D) over tilde T + Delta (E) over tilde/RT)]The quantities alpha, beta, (B) over tilde, (D) over tilde and Delta (E) over tilde are described in the text below. A simple substantiation of the formula was also given. This relation gives immediately the Mooney approximation and allows the prediction of the values of the parameter S and a self-crowding factor K in this approximation. By applying an asymptotic form of the formula such rheological quantities as the intrinsic viscosity and Huggins coefficient were calculated.