Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae.
Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvae.
复制标题
胚胎暴露于五溴苯会抑制斑马鱼幼鱼后部鱼鳔的膨胀。
DOI：
10.1016/j.envpol.2020.113923
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发表时间：
2020
影响因子：
8.9
通讯作者：
Bingsheng Zhou
中科院分区：
文献类型：
--
作者：
W. Peng;Sitian Liu;Yongyong Guo;Lihua Yang;Bingsheng Zhou
五溴代苯(PBB)是一种新兴的阻燃剂，近年来经常被检测到，并可能对野生动物和人类构成暴露风险。本研究以斑马鱼仔鱼后部鱼囊的充气为终点，研究了多溴联苯的发育毒性及其可能的作用机制。结果表明，胚胎暴露于PBB可显著抑制后部鱼囊的充气。在120hpf时，经PBB处理的斑马鱼幼体中的T3水平降低，并出现了crhandpomcc的转录变化。而参与合成(促甲状腺激素)、生物转化(uGt1ab、dio2)和功能调节(trα、trβ、gr)的甲状腺和肾上腺皮质系统的关键调节因子无明显变化。进一步的数据显示，prlra是在受精后96小时检测到的基因中唯一发生改变的基因。在120hpf时，0.25hpf或更高的μM处理组的鱼囊形态表现为放气。此外，在120hpf时，axa5、prlra、prlrb、atp1b2和slc12a10的mRNA水平均发生显著变化。综上所述，我们推测胚胎暴露于PBB抑制了斑马鱼鱼囊的膨胀，这可能是通过介导的途径实现的。观察到的甲状腺和肾上腺皮质参数的变化可能是PBB暴露引起的间接影响。总体而言，我们的研究结果为未来新型阻燃剂PBB的毒理学研究和风险评估提供了重要的数据和迹象。
The emerging flame retardants pentabromobenzene (PBB) has been frequently detected in recent years and may pose exposure risks to wild animals and human beings. In this study, the inflation of posterior swim bladder of zebrafish larvae was used as an endpoint to study the developmental toxicity and putative mechanisms associated with PBB toxicity. Our results showed that embryonic exposure to PBB could significantly inhibit the inflation of posterior swim bladders. Reduced T3 levels and transcriptional changes ofcrhandpomcwere observed in PBB treated zebrafish larvae at 120 hpf. However, key regulators of thyroid and adrenocortical system involved in the synthesis (tsh), biological conversion (ugt1ab,dio2) and functional regulation (trα,trβ,gr) showed no significant changes. Further data revealed thatprlrawas the only gene that was altered among the detected genes at 96 h post fertilization (hpf). At 120 hpf, the morphology of swim bladder indicated deflation in treatments at 0.25 μM and higher. In addition, the mRNA levels ofanxa5,prlra,prlrb,atp1b2andslc12a10were all significantly changed at 120 hpf. Taken together, we suppose that embryonic exposure to PBB inhibited the inflation of swim bladder in zebrafish probably viaprlramediated pathways. The observed changes of thyroid and adrenocortical parameters might be indirect effects evoked by PBB exposure. Overall, our results provide important data and indications for future toxicological study and risk assessment of the emerging flame retardants PBB.