Desmoglein‐3 is a target autoantigen in spontaneous canine pemphigus vulgaris
Desmoglein‐3 is a target autoantigen in spontaneous canine pemphigus vulgaris
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Desmoglein-3 是自发性犬寻常型天疱疮的靶自身抗原
DOI：
10.1046/j.0906-6705.2002.072.x
复制
发表时间：
2003
影响因子：
3.6
通讯作者：
R. Ghohestani
中科院分区：
文献类型：
--
作者：
T. Olivry;S. Joubeh;S. Dunston;T. Nishiyama;R. Ghohestani
翻译后摘要：寻常型天疱疮（PV）是一种自身免疫性起泡皮肤病的人类和伴侣动物。在人类患者中，PV与特异性针对钙粘蛋白家族的角质形成细胞桥粒糖蛋白的IgG自身抗体的产生相关。本研究旨在确定PV犬的皮肤和血清中是否存在抗角质形成细胞IgG自身抗体，并确定循环自身抗体靶向的犬PV自身抗原。由于显微镜下显示基底上上皮棘层松解，选择了11只犬。直接免疫荧光显示存在IgG自身抗体结合的角质形成细胞的膜在皮肤活检标本的89只狗（89%）。使用间接免疫荧光法，分别在10/11（91%）和5/11（45%）只犬中发现了血清循环IgG自身抗体，使用正常犬牙龈和培养的犬口腔角质形成细胞。通过使用培养的犬口腔角质形成细胞蛋白裂解物进行免疫印迹，7/9（78%）受试犬的IgG自身抗体识别130-kDa抗原，该抗原与兔抗桥粒芯糖蛋白-3多克隆抗体鉴定的抗原共同迁移。通过免疫沉淀-免疫印迹法证实该130 kDa抗原代表人桥粒芯糖蛋白-3的犬等效物。这些研究的结果提供了证据，证明犬桥粒芯糖蛋白-3同源物是PV犬的主要自身抗原。这些观察结果进一步确立了自发性犬PV作为研究人类这种疾病的发病机制、病因学和新治疗方法的天然模型。
Abstract: Pemphigus vulgaris (PV) is an autoimmune blistering skin disease of humans and companion animals. In human patients, PV is associated with the production of IgG autoantibodies specific for keratinocyte desmosomal glycoproteins of the cadherin family. The purpose of this study was to determine whether antikeratinocyte IgG autoantibodies were present in the skin and serum of dogs with PV, and also to identify the canine PV autoantigen(s) targeted by circulating autoantibodies. Eleven dogs were selected because of the microscopic demonstration of suprabasal epithelial acantholysis. Direct immunofluorescence revealed the presence of IgG autoantibodies bound to the membrane of keratinocytes in skin biopsy specimens of 8/9 dogs (89%). Using indirect immunofluorescence, serum‐circulating IgG autoantibodies were found in 10/11 (91%) and 5/11 (45%) dogs, using normal canine gingiva and cultured canine oral keratinocytes, respectively. By immunoblotting using cultured canine oral keratinocyte protein lysates, IgG autoantibodies from 7/9 (78%) tested dogs recognized a 130‐kDa antigen that comigrated with that identified by rabbit polyclonal antibodies raised against desmoglein‐3. This 130 kDa antigen was confirmed to represent the canine equivalent of human desmoglein‐3 by immunoprecipitation‐immunoblotting. The results of these studies provide evidence that the canine desmoglein‐3 homologue is a major autoantigen in dogs with PV. These observations further establish spontaneous canine PV as a natural model for research on pathogenesis, etiology and novel therapeutic approaches for this disease of humans.
DOI：
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发表时间：
2000
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ghohestani;B. Hudson;A. Claudy;J. Uitto
通讯作者：
R. Ghohestani;B. Hudson;A. Claudy;J. Uitto
天疱疮：故事还有另一半吗？
DOI：
10.1046/j.1523-1747.2001.01307.x
发表时间：
2001
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Stanley,JR;Nishikawa,T;Diaz,LA;Amagai,M
通讯作者：
Amagai,M
体外鳞状癌细胞系对甲状旁腺激素相关蛋白产生的调节。
DOI：
--
发表时间：
1993
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Merryman,JI;Capen,CC;McCauley,LK;Werkmeister,JR;Suter,MM;Rosol,TJ
通讯作者：
Rosol,TJ