Glucose-stimulated insulin secretion suppresses hepatic triglyceride-rich lipoprotein and apoB production
Glucose-stimulated insulin secretion suppresses hepatic triglyceride-rich lipoprotein and apoB production
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DOI：
10.1152/ajpendo.2000.279.5.e1003
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发表时间：
2000-11-01
影响因子：
5.1
通讯作者：
Sparks, JD
中科院分区：
文献类型：
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作者：
Chirieac, DV;Chirieac, LR;Sparks, JD
本研究评估了胰岛素对大鼠肝脏富脂蛋白（TRL）产生的体内影响。在静脉注射Triton WR-1339（400 mg/kg）的麻醉、禁食大鼠中测量肝脏甘油三酯和载脂蛋白B（apo B）的产生。用去污剂处理阻断血管内钙通道后，注射葡萄糖（500 mg/kg）以引起胰岛素分泌，并在接下来的3 h内监测血清甘油三酯和apoB的积累。在注射葡萄糖的大鼠中，甘油三酯分泌平均为22.5 +/- 2.1 mug.ml（-1）.min（-1），比注射生理盐水的大鼠中观察到的平均值32.1 +/- 1.4 μ g. ml（-1）. min（-1）显著减少30%。在注射葡萄糖的大鼠中，ApoB分泌也显著减少了66%。ApoB免疫印迹表明，B100和B48的生产显着减少后，葡萄糖注射。结果支持胰岛素在体内抑制肝脏极低密度脂蛋白（VLDL）甘油三酯和载脂蛋白B分泌的结论，并加强了胰岛素在餐后VLDL代谢中的调节作用的概念。
The current study assessed in vivo the effect of insulin on triglyceride-rich lipoprotein (TRL) production by rat liver. Hepatic triglyceride and apolipoprotein B (apoB) production were measured in anesthetized, fasted rats injected intravenously with Triton WR-1339 (400 mg/kg). After intravascular catabolism was blocked by detergent treatment, glucose (500 mg/kg) was injected to elicit insulin secretion, and serum triglyceride and apoB accumulation were monitored over the next 3 h. In glucose-injected rats, triglyceride secretion averaged 22.5 +/- 2.1 mug.ml(-1).min(-1), which was significantly less by 30% than that observed in saline-injected rats, which averaged 32.1 +/- 1.4 mug.ml(-1)min(-1). ApoB secretion was also significantly reduced by 66% in glucose-injected rats. ApoB immunoblotting indicated that both B100 and B48 production were significantly reduced after glucose injection. Results support the conclusion that insulin acts in vivo to suppress hepatic very low density lipoprotein (VLDL) triglyceride and apoB secretion and strengthen the concept of a regulatory role for insulin in VLDL metabolism postprandially.