The capsule of human Meissner corpuscles: immunohistochemical evidence
The capsule of human Meissner corpuscles: immunohistochemical evidence
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DOI：
10.1111/joa.13139
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发表时间：
2019-12-22
影响因子：
2.4
通讯作者：
Vega, Jose A.
中科院分区：
文献类型：
--
作者：
Garcia-Piqueras, Jorge;Cobo, Ramon;Vega, Jose A.
迈斯纳小体是一种皮肤机械感受器，通常位于人无毛皮肤的真皮乳头。在结构上，这些感觉小体由机械感受性感觉神经元组成，周围是无髓鞘层状雪旺样细胞。一些作者描述了迈斯纳小体周围部分发育的神经内膜或神经周围起源的纤维母细胞囊；然而，其他人注意到这些结构是非封装的。由于形成感觉小体的轴周细胞与神经干细胞之间存在连续性，我们使用免疫组织化学方法检测了人皮肤迈斯纳小体中神经内膜（CD34抗原）或神经周围[葡萄糖转运蛋白1 (Glut1)]标记物的表达。我们还研究了迈斯纳小体中巢蛋白和脉蛋白（分别是神经内膜细胞和神经周围细胞骨架的主要中间纤维）的免疫组织化学模式。本研究最重要的发现是cd34阳性细胞在大多数迈斯纳小体周围形成部分/完全神经内膜起源的囊，没有其他神经周围glut1阳性元素的迹象。然而，囊膜cd34阳性细胞的细胞骨架蛋白不包括巢蛋白或静脉蛋白，因此这些细胞的细胞骨架组成仍有待确定。最后，囊膜中CD34的免疫反应强度随着年龄的增长而下降，有时在最年长的个体中完全没有。总之，我们报告了人类迈斯纳小体胶囊的第一个免疫组织化学证据，并证明了胶囊的神经内膜起源。
Meissner corpuscles are cutaneous mechanoreceptors that are usually located in the dermal papillae of human glabrous skin. Structurally, these sensory corpuscles consist of a mechanoreceptive sensory neuron surrounded by non-myelinating lamellar Schwann-like cells. Some authors have described a partially developed fibroblastic capsule of endoneurial or perineurial origin around Meissner corpuscles; however, others have noted that these structures are non-encapsulated. As there is continuity between the periaxonic cells forming the sensory corpuscles and the cells of the nerve trunks, we used immunohistochemistry to examine the expression of endoneurial (CD34 antigen) or perineurial [Glucose transporter 1 (Glut1)] markers in human cutaneous Meissner corpuscles. We also investigated the immunohistochemical patterns of nestin and vimentin (the main intermediate filaments of the cytoskeleton of endoneurial and perineurial cells, respectively) in Meissner corpuscles. The most important finding from this study was that CD34-positive cells formed a partial/complete capsule of endoneurial origin around most Meissner corpuscles, without signs of other perineurial Glut1-positive elements. However, the cytoskeletal proteins of the capsular CD34-positive cells did not include either nestin or vimentin, so the cytoskeletal composition of these cells remains to be established. Finally, the intensity of the immunoreactivity for CD34 in the capsule decreased with ageing, sometimes becoming completely absent in the oldest individuals. In conclusion, we report the first immunohistochemical evidence of the capsule of Meissner corpuscles in humans and demonstrate the endoneurial origin of the capsule.