BETA CELLS ARISE FROM GLUCOSE-TRANSPORTER TYPE-2 (GLUT2)-EXPRESSING EPITHELIAL-CELLS OF THE DEVELOPING RAT PANCREAS
BETA CELLS ARISE FROM GLUCOSE-TRANSPORTER TYPE-2 (GLUT2)-EXPRESSING EPITHELIAL-CELLS OF THE DEVELOPING RAT PANCREAS
复制标题
DOI：
10.1073/pnas.91.20.9559
复制
发表时间：
1994-09-27
影响因子：
11.1
通讯作者：
WONG, GG
中科院分区：
文献类型：
--
作者：
PANG, K;MUKONOWESHURO, C;WONG, GG
我们观察Glut2蛋白在第11天（E11）大鼠胚胎中的一个endodesmal域包含胰腺原基。随着内胚层上皮外翻到周围间充质中以形成胰腺芽，Glut2表达继续。背侧和腹侧胰腺芽的细胞在上皮生长和分支形成导管时保持Glut2表达。随着腺泡在导管末端形成，腺泡细胞停止Glut2表达。胰岛素蛋白首先在胰腺上皮和间充质之间的界面处的小簇中检测到。这些簇在E13和E17之间分散到间质组织中。在E17时，在表达Glut2的导管网络中出现了一个独特的、更大的胰岛素表达细胞群。然后，胰岛素和Glut2共表达细胞似乎分离成大的聚集体，形成胰岛的β细胞。这些观察结果支持这一假设，即在胰腺形成过程中出现两种生物学上不同的胰岛素表达细胞群。
We observe Glut2 protein in day 11 (E11) rat embryos in an erndodesmal domain containing the pancreatic primordium. Glut2 expression continues as the endodermal epithelium evaginates into the surrounding mesenchyme to form the pancreatic buds. Cells of the dorsal and ventral pancreatic buds maintain Glut2 expression as the epithelium grows and branches to form ducts. As acini form at the ends of the ducts, acinar cells cease Glut2 expression. Insulin protein is first detected in small clusters at the interface between pancreatic epithelium and mesenchyme. These clusters disperse into the interstitial tissue between E13 and E17. At E17 a distinct, larger population of insulin-expressing cells arises in the Glut2-expressing ductal network. Insulin- and Glut2-coexpressing cells then appear to segregate into large aggregates to form the beta cells of the islets of Langerhans. These observations support the hypothesis that two biologically distinct populations of insulin-expressing cells arise during pancreas formation.