NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.
NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.
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使用新型时间选通 SPAD 相机进行近红外荧光寿命宏观成像。
DOI：
10.1117/12.2649227
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Charbon,Edoardo
中科院分区：
文献类型：
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作者：
Michalet,Xavier;Ulku,ArinC;Wayne,MichaelA;Weiss,Shimon;Bruschini,Claudio;Charbon,Edoardo
SwissSPAD 3是为荧光寿命成像（FLI）应用开发的宽视场时间选通SPAD成像仪系列中的最新产品。其独特的特点是：（i）能够定义比其前身更短的门（宽度W < 1 ns），（ii）支持至少80 MHz的激光重复率，以及（iii）提供100%有效占空比的双门架构。我们提出了宽场宏观FLI测量短寿命近红外染料，分析使用相量的方法。结果与以前获得的SwissSPAD 2和理论预测。
SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (widthW< 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.