Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices
Cell detection and counting through cell lysate impedance spectroscopy in microfluidic devices
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DOI：
10.1039/b705082h
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发表时间：
2007-06-01
期刊：
影响因子：
6.1
通讯作者：
Bashir, Rashid
中科院分区：
文献类型：
--
作者：
Cheng, Xuanhong;Liu, Yi-shao;Bashir, Rashid
基于细胞的微流体装置已经吸引了广泛应用的兴趣。虽然光学细胞计数和流式细胞仪类型的设备已被广泛报道，但通常缺乏检测和量化附着在微型设备内的大表面积上的细胞的灵敏且有效的非光学方法。我们描述了一种基于测量周围介质的电导率变化的电方法，该电导率变化是由于从微流体通道内的表面固定化细胞释放的离子引起的。使用低电导率、低渗培养基溶解固定化细胞，并使用表面图案电极测量由此产生的阻抗变化，以检测和量化细胞数量。我们发现，本体溶液电导线性增加的孤立的细胞的数量有助于溶液离子浓度。细胞裂解物阻抗谱的方法是足够灵敏的检测20个细胞μ L-1，并提供了一个简单而有效的方法，用于检测和计数的许多应用，包括在资源有限的环境中的HIV患者的CD 4细胞计数的测量微流体装置内的细胞。据我们所知，这是使用非光学装置来计数固定化细胞的最灵敏的方法。能够从复杂的生物流体中分离特定细胞类型并量化细胞数量的微流体装置可以用作手持式仪器的一次性盒，以在护理点设置中提供简单、快速和负担得起的细胞计数。
Cell-based microfluidic devices have attracted interest for a wide range of applications. While optical cell counting and flow cytometry-type devices have been reported extensively, sensitive and efficient non-optical methods to detect and quantify cells attached over large surface areas within microdevices are generally lacking. We describe an electrical method for counting cells based on the measurement of changes in conductivity of the surrounding medium due to ions released from surface-immobilized cells within a microfluidic channel. Immobilized cells are lysed using a low conductivity, hypotonic media and the resulting change in impedance is measured using surface patterned electrodes to detect and quantify the number of cells. We found that the bulk solution conductance increases linearly with the number of isolated cells contributing to solution ion concentration. The method of cell lysate impedance spectroscopy is sensitive enough to detect 20 cells mu L-1, and offers a simple and efficient method for detecting and enumerating cells within microfluidic devices for many applications including measurement of CD4 cell counts in HIV patients in resource-limited settings. To our knowledge, this is the most sensitive approach using non-optical setups to enumerate immobilized cells. The microfluidic device, capable of isolating specific cell types from a complex bio-fluidic and quantifying cell number, can serve as a single use cartridge for a hand-held instrument to provide simple, fast and affordable cell counting in point-of-care settings.