Nanomechanical Probes of Single Corneal Epithelial Cells: Shear Stress and Elastic Modulus
Nanomechanical Probes of Single Corneal Epithelial Cells: Shear Stress and Elastic Modulus
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单角膜上皮细胞的纳米机械探针：剪切应力和弹性模量
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Scott S. Perry
中科院分区：
文献类型：
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作者：
Joelle P. Straehla;F. T. Limpoco;N. Dolgova;B. Keselowsky;W. Sawyer;Scott S. Perry
利用原子力显微镜对活体人角膜上皮细胞进行了体外探测，揭示了单个细胞的摩擦特性。在细胞介质中，测量的剪应力为0.40kpa，表明与微球探针接触的上皮细胞表面具有高度的润滑性。通过纳米尺度的压痕测量，进一步探讨了单个上皮细胞的机械特性。一个简单的弹性基础模型，基于实验可验证的参数，用于拟合压痕数据，产生16.5千帕的有效弹性模数，并突出细胞表面的高度顺应性。发现弹性地基模型更准确地拟合了实验数据，避免了不可验证的假设，并产生了比广泛使用的Hertz-Sneddon模型高得多的模数。
Living human corneal epithelial cells have been probed in vitro via atomic force microscopy, revealing the frictional characteristics of single cells. Under cell media, measured shear stresses of 0.40 kPa demonstrate the high lubricity of epithelial cell surfaces in contact with a microsphere probe. The mechanical properties of individual epithelial cells have been further probed through nanometer scale indentation measurements. A simple elastic foundation model, based on experimentally verifiable parameters, is used to fit the indentation data, producing an effective elastic modulus of 16.5 kPa and highlighting the highly compliant nature of the cell surface. The elastic foundation model is found to more accurately fit the experimental data, to avoid unverifiable assumptions, and to produce a modulus significantly higher than that of the widely used Hertz–Sneddon model.
影响因子：
3.4
作者：
Dimitriadis, EK;Horkay, F;Chadwick, RS
通讯作者：
Chadwick, RS
影响因子：
2.4
作者：
Park, S;Costa, KD;Ateshian, GA
通讯作者：
Ateshian, GA