Lymphocyte Populations in Joint Tissues from Dogs with Inflammatory Stifle Arthritis and Associated Degenerative Cranial Cruciate Ligament Rupture
Lymphocyte Populations in Joint Tissues from Dogs with Inflammatory Stifle Arthritis and Associated Degenerative Cranial Cruciate Ligament Rupture
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DOI：
10.1111/j.1532-950x.2011.00867.x
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发表时间：
2011-08-01
影响因子：
1.8
通讯作者：
Plisch, Erin
中科院分区：
文献类型：
--
作者：
Muir, Peter;Kelly, Jennifer L.;Plisch, Erin
目的：评价退行性颅交叉韧带断裂（CCLs）犬后膝关节滑膜和滑液中的淋巴细胞群。研究设计：前瞻性临床研究。动物：患有后膝关节关节炎和CCLs的犬（n = 25），7只患有与软骨退行性变相关的关节炎（骨关节炎[OA]）的犬，以及12只具有完整CCLs的健康Beagle犬。收集关节组织后，分离单个核细胞，随后使用流式细胞术分析CD 3、CD 4、CD 8和CD 21的表达。CD 4（+）辅助性T淋巴细胞、CD 8（+）细胞毒性T淋巴细胞、与健康Beagle犬相比，慢性阻塞性肺疾病犬滑膜中CD 3（+）、CD 4（-）、CD 8（-）T淋巴细胞数量增加（P <0.05）。与OA犬相比，CCR 4犬滑液中CD 3（+）CD 4（-）CD 8- T淋巴细胞比例增加（P <0.05）。结论：膝关节滑膜及关节液中的淋巴细胞性炎症是膝关节炎的重要特征。淋巴细胞群包括表达CD 4和CD 8的T淋巴细胞，以及CD 3（+）CD 4（-）CD 8（-）T淋巴细胞。CD 3（+）CD 4（-）CD 8（-）T淋巴细胞的存在与后膝关节滑膜炎的发生有关。需要进一步的工作来完全鉴定这些细胞的表型。
Objective: To evaluate lymphocyte populations in stifle synovium and synovial fluid of dogs with degenerative cranial cruciate ligament rupture (CCLR).Study Design: Prospective clinical study.Animals: Dogs (n = 25) with stifle arthritis and CCLR, 7 dogs with arthritis associated with cartilage degeneration (osteoarthritis [OA]), and 12 healthy Beagle dogs with intact CCL.Methods: Arthritis was graded radiographically in CCLR dogs. After collection of joint tissues, mononuclear cells were isolated and subsequently analyzed using flow cytometry for expression of CD3, CD4, CD8, and CD21.Results: The proportions of CD4(+) T helper lymphocytes, CD8(+) cytotoxic T lymphocytes, and CD3(+) CD4(-) CD8(-) T lymphocytes were increased in synovium from dogs with CCLR compared with synovium from healthy Beagle dogs (P < .05). The proportion of CD3(+) CD4(-) CD8- T lymphocytes in synovial fluid was increased in dogs with CCLR compared with dogs with OA (P < .05). In dogs with CCLR, the proportion of CD3(+) CD4(-) CD8(-) T lymphocytes in synovial fluid was inversely correlated with radiographic arthritis (SR= -0.68, P < .005).Conclusion: Lymphocytic inflammation of stifle synovium and synovial fluid is an important feature of the CCLR arthropathy. Lymphocyte populations include T lymphocytes expressing CD4 and CD8, and CD3(+) CD4(-) CD8(-) T lymphocytes. Presence of CD3(+) CD4(-) CD8(-) T lymphocytes was associated with development of stifle synovitis. Further work is needed to fully identify the phenotype of these cells.