VEGF upregulates VEGF receptor-2 on human outer root sheath cells and stimulates proliferation through ERK pathway
VEGF upregulates VEGF receptor-2 on human outer root sheath cells and stimulates proliferation through ERK pathway
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VEGF 上调人外根鞘细胞上的 VEGF 受体 2，并通过 ERK 通路刺激增殖
DOI：
10.1007/s11033-012-1725-6
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发表时间：
2012-09-01
影响因子：
2.8
通讯作者：
Zheng, Min
中科院分区：
文献类型：
--
作者：
Li, Wei;Lu, Zhong-Fa;Zheng, Min
血管内皮生长因子（VEGF）是生理和病理血管生成的关键调节因子。VEGF的生物学效应是由受体酪氨酸激酶介导的。VEGF受体-2是VEGF的主要受体，被认为介导了大多数功能效应。在本研究中，我们检测了VEGF受体-2在人外根鞘细胞（ORS）中的表达及其作用。采用逆转录聚合酶链反应（RT-PCR）和Western blot检测vegf -2 mRNA和蛋白水平的表达。免疫荧光法检测VEGFR-2在ORS细胞中的定位。MTT法检测VEGF对ORS细胞增殖的影响。我们的数据显示，VEGFR-2在ORS细胞中的mRNA和蛋白水平均有表达。VEGFR-2免疫染色在培养的ORS细胞上显示出较强的信号。外源性vegf165刺激ORS细胞增殖，并以剂量依赖性方式上调VEGFR-2的表达。此外，vegf165以时间依赖性的方式诱导VEGFR-2、PLC-γ1、PKC-α、MEK和p44/42 MAPK （ERK1/2）的磷酸化。综上所述，人类ORS细胞表达功能性VEGF受体-2，外源性vegf165通过VEGFR-2介导的ERK信号通路上调VEGFR-2的表达，刺激ORS细胞增殖。
Vascular endothelial growth factor (VEGF) is a key regulator of physiological and pathological angiogenesis. The biological effects of VEGF are mediated by receptor tyrosine kinases. VEGF receptor-2, the primary receptor for VEGF, is thought to mediate most functional effects. In this study, we examined the expression and roles of VEGF receptor-2 on human outer root sheath cells (ORS). The expression of VEGFR-2 was determined at mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Localization of VEGFR-2 in ORS cells was detected by immunofluorescence. The effect of VEGF on ORS cell proliferation was determined by MTT assays. Our data showed the expression of VEGFR-2 on ORS cells at both mRNA and protein levels. Immunostaining for VEGFR-2 demonstrated strong signal on cultured ORS cells. Exogenous VEGF165stimulated proliferation of ORS cells and upregulated expression of VEGFR-2 in a dose-dependent manner. Moreover, VEGF165induced phosphorylation of VEGFR-2, PLC-γ1, PKC-α, MEK, and p44/42 MAPK (ERK1/2) in a time-dependent manner. Taken together, human ORS cells express functional VEGF receptor-2 and exogenous VEGF165upregulates expression of VEGFR-2 and stimulates proliferation of ORS cells via VEGFR-2 mediated ERK signaling pathway.