Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA
Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA
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DOI：
10.1088/1361-6528/abef2c
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发表时间：
2021-03
期刊：
影响因子：
3.5
通讯作者：
Hiromi Takahashi;T. Yasui;H. Kashida;Koki Makino;Keiko Shinjo;Quanli Liu;Taisuke Shimada;S. Rahong;N. Kaji;H. Asanuma;Y. Baba
中科院分区：
文献类型：
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作者：
Hiromi Takahashi;T. Yasui;H. Kashida;Koki Makino;Keiko Shinjo;Quanli Liu;Taisuke Shimada;S. Rahong;N. Kaji;H. Asanuma;Y. Baba
无细胞DNA(CfDNA)的检测对液体活检中的DNA分析有影响。然而，目前检测cfDNA的策略有一些局限性需要克服，例如灵敏度低，需要大量的时间和专门的仪器。因此，疾病预防和早期治疗需要非侵入性和快速的检测工具。在这里，我们开发了一种基于DNA探针杂交的微加热器与氧化锌纳米线集成的装置，用于检测目标单链DNA(SsDNA)。我们在实验上证实，我们的装置实现了DNA探针的原位退火，随后我们利用该探针检测到了目标单链DNA。我们设想，该装置可用于与基于纳米生物装置的DNA检测相关的基础研究。
Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.