Relationship between lymphangiogenesis and exudates during the wound-healing process of mouse skin full-thickness wound
Relationship between lymphangiogenesis and exudates during the wound-healing process of mouse skin full-thickness wound
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DOI：
10.1111/j.1524-475x.2009.00512.x
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Okuwa, Mayumi
中科院分区：
文献类型：
--
作者：
Shimamura, Kimi;Nakatani, Toshio;Okuwa, Mayumi
我们考虑了伤口愈合过程中渗出液、伤口面积、血管生成、淋巴管生成和上皮再生之间的关系。在小鼠背部制作全层伤口。每天测定吸收到敷料中的渗出液重量以及伤口面积。用抗LYVE-1和CD 31抗体对伤口切片进行染色。于伤后第3、5、7天将墨汁注入伤口，观察渗出液的移动情况。在第11天，新上皮完全覆盖伤口。渗出液量在第1天达到峰值，然后迅速下降，直至第11天无法检测到。注射到伤口中的大部分印度墨水保留在伤口内，没有流入周围组织。第5天新生血管在肉芽组织中均匀分布。肉芽组织中的新血管比血管晚约2天出现，并且在第11天它们向肉芽组织的中心分布。因此，伤口渗出液的减少似乎与血管有关，而与血管无关。然而，增加促炎剂可能在伤口愈合过程的后期发挥作用。
We considered the relationship among exudate, wound area, angiogenesis, lymphangiogenesis, and reepithelialization during wound healing. Full-thickness wounds were made on the dorsum of mice. The weight of exudate absorbed into the dressing as well as the wound area was determined daily. Sections of the wounds were stained with anti-LYVE-1 and CD31 antibodies. Indian ink was injected into the wound for observing the movement of the exudate on days 3, 5, and 7 after wounding. New epithelium completely covered the wound on day 11. The quantity of exudate peaked on day 1, and then rapidly decreased until it was undetectable on day 11. Most of the Indian ink injected into the wound was retained within the wound and did not flow into the surrounding tissue. New blood vessels showed a uniform distribution in the granulation tissue on day 5. New lymphatics appeared in the granulation tissue approximately 2 days later than the blood vessels and they were distributed toward the center of the granulation tissue on day 11. Thus, reduction of exudate from the wound appears to be related to blood vessels, not lymphatics. However, increasing lymphatics may play a role in the late phase of the wound-healing process.