HEPARITINASE INHIBITION OF MESODERM INDUCTION AND GASTRULATION IN XENOPUS-LAEVIS EMBRYOS
HEPARITINASE INHIBITION OF MESODERM INDUCTION AND GASTRULATION IN XENOPUS-LAEVIS EMBRYOS
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DOI：
10.1006/dbio.1994.1218
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发表时间：
1994-08-01
影响因子：
2.7
通讯作者：
GERHART, JC
中科院分区：
文献类型：
--
作者：
BRICKMAN, MC;GERHART, JC
我们研究了蛋白多糖分子在非洲爪蟾胚胎中中胚层组织诱导中的作用。在囊胚早期向囊胚腔内注射肝素酶可导致原肠形成缺陷和胚胎前部结构发育失败。胚胎对这种处理的敏感性表明这些分子在中胚层诱导过程中可能起作用。研究表明，在爪蟾早期胚胎中，硫酸肝素蛋白聚糖（HSPGs）和硫酸软骨素蛋白聚糖是主要的硫酸糖缀合物，并且HSPGs可以通过胚腔内注射肝素酶降解。此外，使用三个独立的中胚层诱导标准，在爪蟾8期胚胎动物帽组织的外植体中，bFGF的诱导被肝素酶阻断，而不是被软骨素酶ABC阻断。自从hspg出现以来。在用于中胚层诱导实验的培养条件下，囊胚动物帽细胞被肝素酶消化，我们认为细胞表面硫酸肝素蛋白聚糖是碱性成纤维细胞生长因子介导的中胚层诱导所必需的。(C)学术出版社
We have examined the involvement of proteoglycan molecules in the induction of mesodermal tissue in Xenopus laevis embryos. Blastocoelic injections of the enzyme heparitinase at early blastula stages lead to gastrulation defects and to failures in the development of anterior embryonic structures. The period of sensitivity of embryos to this treatment suggests a possible role for these molecules during mesoderm induction. We show that heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans are the predominant sulfated glycoconjugates synthesized in early Xenopus embryos and that HSPGs are degraded by blastocoelic injections of heparitinase. Further, bFGF induction of mesoderm in explants of Xenopus stage 8 embryonic animal cap tissue is blocked by heparitinase but not by Chondroitinase ABC, using three separate criteria of mesoderm induction. Since HSPGs present. in blastula animal cap cells are digested by heparitinase under the culture conditions used in the mesoderm-induction assay, we suggest that cell-surface heparan sulfate proteoglycans are required for basic fibroblast growth factor-mediated mesoderm induction. (C) Academic Press, Inc.