The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development.
The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development.
复制标题
番茄花同源异型蛋白 FBP1 样基因 SlGLO1 在花瓣和雄蕊发育中发挥关键作用
DOI：
10.1038/srep20454
复制
发表时间：
2016-02-04
影响因子：
4.6
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Guo X;Hu Z;Yin W;Yu X;Zhu Z;Zhang J;Chen G
MADS-box 转录因子在植物生长和发育中发挥着重要作用，尤其是花器官特征。在我们的研究中，分离出一个MADS-box基因SlGLO1-番茄花同源异型蛋白FBP1样基因。其组织特异性表达谱分析表明SlGLO1在花瓣和雄蕊中高表达。 SlGLO1 的 RNAi（RNA 干扰）抑制导致花器官异常表型，包括尺寸较短的绿色花瓣和异常的心皮雄蕊。 SlGLO1 沉默系是雄性不育的。 SlGLO1沉默的花瓣和雄蕊中总叶绿素含量增加，叶绿素生物合成基因显着上调。此外，B类基因表达分析表明，在SlGLO1-RNAi植物中，SlGLO1功能的抑制导致花瓣和雄蕊中TAP3表达增强，TPI表达下调，而花瓣和雄蕊中TM6表达减少，雄蕊和心皮中TM6表达增加。此外，转基因品系的花粉粒异常且无法发芽，番茄花粉特异性基因在 SlGLO1 沉默品系中下调了 90% 以上。这些结果表明SlGLO1在调节番茄植物花器官和花粉发育中发挥重要作用。
MADS-box transcription factors play important role in plant growth and development, especially floral organ identities. In our study, a MADS-box gene SlGLO1- tomato floral homeotic protein FBP1-like gene was isolated. Its tissue-specific expression profile analysis showed that SlGLO1 was highly expressed in petals and stamens. RNAi (RNA interference) repression of SlGLO1 resulted in floral organ abnormal phenotypes, including green petals with shorter size and aberrant carpelloid stamens. SlGLO1-silenced lines are male sterile. Total chlorophyll content was increased and chlorophyll biosynthetic genes were significantly up-regulated in SlGLO1-silenced petals and stamens. Furthermore, B-class genes expression analysis indicated that the repressed function of SlGLO1 led to the enhanced expression of TAP3 and the down-regulation of TPI in the petals and stamens, while the expression of TM6 was reduced in petals and increased in stamens and carpels of SlGLO1-RNAi plants. Additionally, pollen grains of transgenic lines were aberrant and failed to germinate and tomato pollen-specific genes were down-regulated by more than 90% in SlGLO1-silenced lines. These results suggest that SlGLO1 plays important role in regulating plant floral organ and pollen development in tomato.