An Alarmingly High Proportion of HIV-1 Isolates Carrying Mutations Corresponding to Resistance to Antiretroviral Drugs among HIV-Positive High-Risk Groups in Central Vietnam: a Substudy of the National Sentinel Survey
An Alarmingly High Proportion of HIV-1 Isolates Carrying Mutations Corresponding to Resistance to Antiretroviral Drugs among HIV-Positive High-Risk Groups in Central Vietnam: a Substudy of the National Sentinel Survey
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DOI：
10.7883/yoken.jjid.2016.512
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发表时间：
2017-11-01
影响因子：
2.2
通讯作者：
Yoshida, Lay Myint
中科院分区：
文献类型：
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作者：
Hung Thai Do;Dong Thanh Nguyen;Yoshida, Lay Myint
我们研究了越南中部地区高危人群中艾滋病毒耐药性的流行情况，如注射毒品使用者（IDUs）、女性性工作者（FSW）和男男性行为者（MSM）。我们使用了2012-2013年哨点监测调查的艾滋病毒阳性血液样本。通过标准化筛选试验对研究受试者进行HIV感染筛查，并通过内部试验对HIV阳性样本进一步检测HIV病毒载量和耐药突变（DRM）。使用斯坦福大学在线序列分析工具鉴定DRM。并对他们的危险行为进行了调查。在研究期间，对6，016名（高危）受试者进行了筛查，97名受试者的艾滋病毒感染检测呈阳性（注射吸毒者：n = 63，3.0%; FSW：n = 24，0.9%; MSM：n = 10，1.0%）。97个样本中有92个（45个来自2012年，47个来自2013年）可供进一步测试。在92份样本中，56份（60.9%）检测到HIV RNA，40份（71.4%）样本成功进行了耐药基因分型。所有分离株均为CRFOLAE亚型，1例（2.5%）IDU的HIV属于B亚型。13人（32.5%）携带HIV，至少有1个DRM：9名注射吸毒者，1名FSW和3名MSM。因此，越南中部高危人群中的艾滋病毒血清阳性率较低，但在高危人群中观察到高比例的耐药HIV-1分离株。
We studied the prevalence of HIV drug resistance among high-risk groups such as injecting drug users (IDUs), female sex workers (FSWs), and men having sex with men (MSM) in central Vietnam. We used HIV-positive blood samples from 2012-2013 sentinel surveillance surveys. Study subjects were screened for HIV infection by standardized screening assays, and the HIV-positive samples were further tested for HIV viral load and drug-resistance mutations (DRMs) by in-house assays. DRMs were identified using the Stanford University online sequence analysis tool. Their risk behaviors were also investigated. During the study period, 6,016 (high-risk) subjects were screened, and 97 tested positive for HIV infection (IDUs: n = 63, 3.0%; FSWs: n = 24, 0.9%; and MSM: n = 10, 1.0%). Ninety-two of the 97 samples (45 from 2012 and 47 from 2013) were available for further testing. HIV RNA was detected in 56 (60.9%) of the 92 samples, and drug resistance genotyping was successfully performed on 40 (71.4%) samples. All these isolates were subtype CRFOLAE, except for 1 (2.5%) IDU whose HIV belongs to subtype B. Thirteen individuals (32.5%) were carrying HIV with at least 1 DRM: 9 IDUs, 1 FSW, and 3 MSM. Thus, HIV seroprevalence among high-risk individuals in central Vietnam is low, but a high proportion of drug resistant HIV-1 isolates is observed in the high-risk group.