Transmission through the ciliary ganglion of the chick
Transmission through the ciliary ganglion of the chick
复制标题
通过雏鸡的睫状神经节传播
DOI：
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发表时间：
1963
期刊：
影响因子：
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通讯作者：
G. Pilar
中科院分区：
文献类型：
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作者：
A. R. Martin;G. Pilar
在之前的论文中（Martin & Pilar, 1963），提出的证据表明，小鸡睫状神经节中许多突触的传递是由于突触前终末和神经节细胞之间的直接电耦合。证据包括观察到，在这些细胞中，兴奋性突触后电位（e.p.s.p）之前有一个快速的去极化，从而启动突触后动作电位。这种快速去极化或“耦合电位”比e.p.s.p.早1-5-2-0毫秒，因此显然与突触前末端的动作电位一致。它的振幅不受膜极化的影响，也不受足以完全消除e.p.s.p的浓度的d -管curarine chloride （DTC）的影响。来自神经节的突触前末端的记录显示存在类似的偶联电位，这是对神经节后神经的反向刺激产生的反应，表明偶联是双向的。有人认为，这种电耦合与先前在神经节中描述的杯状神经末梢有关（Carpenter, 1911; de Lorenzo, 1960）。偶联电位存在于约60%的细胞中。其余的突触传递是由e.p.s.p介导的。这些被认为与更分散的突触前末端网络或不太发达的钙盏有关。在本实验中，通过刺激突触前和突触后神经干并记录其在神经节中的传递。实验的目的是确定当细胞不受微移液管插入的干扰时，电传递是否有效，如果有效，则估计发生这种传递的突触的比例。
In the preceding paper (Martin & Pilar, 1963), evidence was presented which suggested that transmission at many of the synapses in the ciliary ganglion of the chick was due to direct electrical coupling between presynaptic terminals and ganglion cells. The evidence consisted of the observation that in such cells the excitatory post-synaptic potential (e.p.s.p.) was preceded by a rapid depolarization which initiated the post-synaptic action potential. This rapid depolarization, or 'coupling potential' preceded the e.p.s.p. by 1-5-2-0 msec and was thus apparently coincident with the action potential in the presynaptic terminal. Its amplitude was independent of membrane polarization and was not affected by D-tubocurarine chloride (DTC) in concentrations sufficient to abolish completely the e.p.s.p. Records from presynaptic terminals in the ganglion revealed the presence of a similar coupling potential, produced in response to antidromic stimulation of the post-ganglionic nerve, indicating that the coupling was bi-directional. It was suggested that such electrical coupling was associated with caliciform nerve terminals previously described in the ganglion (Carpenter, 1911; de Lorenzo, 1960). The coupling potential was present in about 60% of the cells studied. In the remainder synaptic transmission was mediated by the e.p.s.p. These were assumed to be associated with more diffuse presynaptic terminal networks or with less well developed calices. In the present experiments transmission through the ganglion was studied by stimulating and recording from the preand postsynaptic nerve trunks. The experiments were carried out to determine whether electrical transmission was effective when the cells were not disturbed by insertion of micropipettes and, if so, to estimate the proportion of synapses at which such transmission occurred.