In Vitro Expression of Benzylamine Oxidase Activity in Cultured Porcine Smooth Muscle Cells
In Vitro Expression of Benzylamine Oxidase Activity in Cultured Porcine Smooth Muscle Cells
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DOI：
10.1097/00005344-198706000-00005
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发表时间：
1987-06
影响因子：
3
通讯作者：
R. M. Hysmith;P. Boor
中科院分区：
文献类型：
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作者：
R. M. Hysmith;P. Boor
在培养的成年猪胸主动脉来源的平滑肌和内皮细胞以及解剖的主动脉内层和内膜层的匀浆中测量了苯甲胺氧化酶（BzAO）活性。 BzAO 似乎位于主动脉内侧匀浆中。培养的平滑肌细胞（通过电子显微镜特征和抗肌球蛋白阳性染色验证）显示出高BzAO活性，在pH 7.0时具有最佳活性；在内皮细胞中未检测到活性。平滑肌细胞连续传代时间延长导致 BzAO 活性增加，其中第 12 次群体倍增时增加最为明显。此外，在第 12 次群体倍增时，BzAO 主要存在于平滑肌细胞培养基的可溶部分中。这些发现支持血管 BzAO 位于内侧平滑肌细胞内的概念，并表明平滑肌细胞可能能够分泌 BzAO。
Benzylamine oxidase (BzAO) activity was measured in cultured smooth muscle and endothelial cells derived from thoracic aorta of adult swine, and in homogenates of dissected aortic medial and intimal layers. BzAO appeared to be localized in aortic medial homogenates. Cultured smooth muscle cells (which were verified by electron microscopic characteristics and positive staining with antimyosin) showed high BzAO activity, with optimal activity at pH 7.0; no activity was detected in endothelial cells. Prolonged serial passage of smooth muscle cells resulted in increased BzAO activity, with the increase most marked by the 12th population doubling. Also, BzAO was found predominantly in the soluble portion of the culture medium of smooth muscle cells by their 12th population doubling. These findings support the concept that vascular BzAO is localized within medial smooth muscle cells, and suggest that smooth muscle cells may be capable of secreting BzAO.