Multimodal imaging reveals a unique autofluorescence signature of Randall's plaque.
Multimodal imaging reveals a unique autofluorescence signature of Randall's plaque.
复制标题
DOI：
10.1007/s00240-020-01216-4
复制
发表时间：
2021-04
期刊：
影响因子：
3.1
通讯作者：
Williams JC Jr
中科院分区：
文献类型：
--
作者：
Winfree S;Weiler C;Bledsoe SB;Gardner T;Sommer AJ;Evan AP;Lingeman JE;Krambeck AE;Worcester EM;El-Achkar TM;Williams JC Jr
肾结石经常发展为在乳头状结缔组织中形成的兰德尔斑块（RP）上的过度生长。RP的有机组成与结石基质不同，因为RP含有纤维状胶原蛋白;组织中的RP也已被证明具有两种在结石中也发现的蛋白质，但RP的分子组成尚未研究。我们假设RP含有独特的有机分子，可以通过荧光与结石过度生长区分开来。为了测试这一点，我们使用微CT引导的抛光暴露肾结石的内部多光子，共聚焦和红外显微镜成像的多模式。我们检测到RP特有的蓝色自体荧光特征，其特异性也在结石患者的乳头组织中得到证实。结石的高分辨率矿物绘图也显示从RP内的磷灰石过渡到过度生长的草酸钙，证明了从组织到尿液的分子和空间过渡。这项工作提供了一个系统的和实用的方法来发现特定的荧光签名与矿物类型，验证以前的观察矿物过度生长到RP和确定一个新的自发荧光签名RP证明RP的独特的分子组成。
Kidney stones frequently develop as an overgrowth on Randall’s plaque (RP) which is formed in the papillary interstitium. The organic composition of RP is distinct from stone matrix in that RP contains fibrillar collagen; RP in tissue has also been shown to have two proteins that are also found in stones, but otherwise the molecular constituents of RP are unstudied. We hypothesized that RP contains unique organic molecules that can be differentiated from the stone overgrowth by fluorescence. To test this, we used micro CT-guided polishing to expose the interior of kidney stones for multimodal imaging with multiphoton, confocal and infrared microscopy. We detected a blue autofluorescence signature unique to RP, the specificity of which was also confirmed in papillary tissue from patients with stone disease. High-resolution mineral mapping of the stone also showed a transition from the apatite within RP to the calcium oxalate in the overgrowth, demonstrating the molecular and spatial transition from the tissue to the urine. This work provides a systematic and practical approach to uncover specific fluorescence signatures which correlate with mineral type, verifies previous observations regarding mineral overgrowth onto RP and identifies a novel autofluorescence signature of RP demonstrating RP’s unique molecular composition.
登录
查看更多内容
影响因子：
3.4
作者：
Williams, James C., Jr.;McAteer, James A.
通讯作者：
McAteer, James A.
影响因子：
3.1
作者：
Sethmann, Ingo;Wendt-Nordahl, Gunnar;Kleebe, Hans-Joachim
通讯作者：
Kleebe, Hans-Joachim
影响因子：
4.8
作者：
Winfree, Seth;Hato, Takashi;Day, Richard N.
通讯作者：
Day, Richard N.
影响因子：
3.1
作者：
O'Kell AL;Lovett AC;Canales BK;Gower LB;Khan SR
通讯作者：
Khan SR
影响因子：
1.6
作者：
Letavernier E;Vandermeersch S;Traxer O;Tligui M;Baud L;Ronco P;Haymann JP;Daudon M
通讯作者：
Daudon M