Expression of ALDH1A Isozymes in Human Endometrium with and without Endometriosis and in Ovarian Endometrioma
Expression of ALDH1A Isozymes in Human Endometrium with and without Endometriosis and in Ovarian Endometrioma
复制标题
DOI：
10.1007/s43032-019-00041-4
复制
发表时间：
2020-01
影响因子：
2.9
通讯作者：
Suke Ma;T. Hirata;Tomoko Arakawa;Hui Sun;Kazuaki Neriishi;Shinya Fukuda;A. Nakazawa;Yu Wang;M. Harada;Yasushi Hirota;K. Koga;O. Wada-Hiraike;T. Fujii;Y. Osuga
中科院分区：
文献类型：
--
作者：
Suke Ma;T. Hirata;Tomoko Arakawa;Hui Sun;Kazuaki Neriishi;Shinya Fukuda;A. Nakazawa;Yu Wang;M. Harada;Yasushi Hirota;K. Koga;O. Wada-Hiraike;T. Fujii;Y. Osuga
子宫内膜是一种高度再生和动态的组织，在月经周期中经历周期性变化。据报道，子宫内膜上皮细胞含有一群祖细胞/干细胞。越来越多的证据表明，祖细胞/干细胞参与了子宫内膜异位症的发病机制。已报道属于醛脱氢酶1（ALDH 1A）家族的蛋白质是正常组织干细胞和癌症干细胞的标志物。在这项研究中，我们使用免疫组织化学，研究ALDH 1A同工酶的表达在人类子宫内膜组织，包括子宫内膜异位症的影响，并在卵巢腺瘤。ALDH 1A同工酶在子宫内膜间质和卵巢异位组织中呈阳性染色。在腺体中，不同的ALDH 1A同工酶的表达模式是不同的。ALDH 1A 1和ALDH 1A 3在子宫内膜的基底层上皮和卵巢腺瘤的上皮中高度表达，而ALDH 1A 2仅在卵巢腺瘤的上皮中高度表达，而与月经周期无关。此外，ALDH 1A 1与N-钙粘蛋白（子宫内膜上皮祖细胞的标志物）共定位于基底层腺体中。这些发现支持并加强了关于基底层和子宫内膜腺体中存在祖细胞/干细胞的概念，表明这些细胞参与了子宫内膜的生理学和子宫内膜异位症的病理学。
Human endometrium is a highly regenerative and dynamic tissue that undergoes cyclic changes during menstrual cycle. It has been reported that endometrial epithelium contains a population of progenitor/stem cells. Increasing amount of evidence indicates that progenitor/stem cells are involved in the pathogenesis of endometriosis. Proteins belonging to the aldehyde dehydrogenase 1 (ALDH1A) family have been reported to be markers of normal tissue stem cells and cancer stem cells. In this study, by using immunohistochemistry, we examined the expression of ALDH1A isozymes in human endometrial tissue, including that affected by endometriosis, and in ovarian endometrioma. Positive staining for ALDH1A isozymes was observed in the stroma of the endometrium and in endometriotic ovarian tissue. In the glands, expression patterns were distinct for different ALDH1A isozymes. ALDH1A1 and ALDH1A3 were highly expressed in the epithelium of stratum basalis of the endometrium and in the epithelium of ovarian endometrioma irrespective of the menstrual cycle, whereas ALDH1A2 was highly expressed only in the epithelium of endometrioma. Furthermore, ALDH1A1 co-localized with N-cadherin, which is a marker of endometrial epithelial progenitor cells, in the glands of stratum basalis. These findings support and reinforce the notion about the presence of progenitor/stem cells in endometrial glands in stratum basalis and in endometriotic glands, suggesting that these cells are involved in the physiology of the endometrium and in the pathology of endometriosis.