Quantitative Trait Locus Mapping of Soybean Maturity Gene E6
Quantitative Trait Locus Mapping of Soybean Maturity Gene E6
复制标题
大豆成熟基因E6的数量性状位点作图
DOI：
10.2135/cropsci2017.02.0106
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发表时间：
2017-09-01
期刊：
影响因子：
2.3
通讯作者：
Kong, Fanjiang
中科院分区：
文献类型：
--
作者：
Li, Xiaoming;Fang, Chao;Kong, Fanjiang
大豆[Glycine max（L.）梅尔。]对光周期的敏感性决定了大豆品种对特定纬度范围的适应性。当适温大豆品种在低纬度短日照条件下种植时，它们开花早，导致谷物产量低，从而限制了它们在热带地区的应用。大多数适应低纬度环境的品种在短日照条件下具有延迟开花的特性，这种特性通常被称为长幼（LJ）。在这项研究中，E6基因座，经典的基因座调节LJ性状，分子定位在Gm 04附近的单核苷酸多态性标记HRM 101。测交、遗传作图和测序表明E6和J位点可能紧密连锁。E6和E1之间的遗传互作评价表明，E6对E1具有抑制作用，并且E6的功能依赖于E1。E6基因的标记对大豆在低纬度环境下的广适性和稳产性的分子育种具有重要意义。E6基因的分子鉴定和功能鉴定将极大地促进对LJ性状的遗传和分子机制的理解。X. Li，C.芳，M. Xu，F. Zhang，S. Lu，H. Nan，T. Su，S. Li，X.赵湖，加-地孔，X.袁，B。刘和F.中国科学院东北地理与农业生态研究所大豆分子设计育种重点实验室，河北省石家庄市哈平路138号，地址：哈尔滨市南岗区南岗路150081 Lu，H. Nan，X.赵，B。Liu和F.广州大学生命科学学院，广东省广州市; X. Li，C. Fang，F. Zhang，T. Su，S. Li和L.中国科学院大学，北京100049;黑龙江省农业科学院，学府路368号，哈尔滨150086; J. Abe，农业研究部，北海道大学，日本北海道札幌060-8589; E.R. Cober，渥太华研究与开发中心，加拿大农业和农业食品，Bldg.#110，中央实验农场，渥太华，ON K1 A 0 C6，加拿大。X. Li，C.芳，M. Xu，F. Zhang和S.卢对这项工作同样作出了贡献。2017年2月18日接收。2017年6月6日接受。* 通讯作者（kongfj@iga.ac.cn; elroy. agr.gc.ca; jabe@res.agr.hokudai.ac.jp; liubh@iga.ac.cn）。致李增禄副主编。缩略语：CJ，常规青少年; DAE，出苗后天数;高分辨率熔化插入缺失、插入和缺失; LD，长日; LJ，长少年; LOD，几率的对数; QTL，数量性状位点; PCR，聚合酶链反应; PGH，Paranagoiana与Harosoy杂交的F2群体; PGI，Paranagoiana与PI 159925杂交的F2群体; PGO，Paranagoiana与OT 94 -47杂交的F2群体; SD，短日照; SNP，单核苷酸多态性; SSR，简单重复序列。发表在Crop Sci。57：2547-2554（2017）。doi：10.2135/cropsci2017.02.0106 ©美国作物科学学会|5585 Guilford Rd.，麦迪逊，威斯康星州53711美国这是一个开放获取的文章下分发的CC BY-NC-ND许可证（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。Published online九月14，2017
Soybean [Glycine max (L.) Merr.] sensitivity to photoperiod determines adaptation to a specific range of latitudes for soybean cultivars. When temperate-adapted soybean cultivars are grown in low latitude under short day conditions, they flower early, resulting in low grain yield, and consequently limiting their utility in tropical areas. Most cultivars adapted to low-latitude environments have the trait of delayed flowering under short day conditions, and this trait is commonly called long juvenile (LJ). In this study, the E6 locus, the classical locus conditioning the LJ trait, was molecularly mapped on Gm04 near single-nucleotide polymorphism marker HRM101. Testcross, genetic mapping, and sequencing suggest that the E6 and J loci might be tightly linked. Genetic interaction evaluation between E6 and E1 suggests that E6 has a suppressive effect on E1 and that the function of E6 is dependent on E1. The tagging markers for E6 are very useful for molecular breeding for wide adaptation and stable productivity of soybean under lowlatitude environments. Molecular identification and functional characterization of the E6 gene will greatly facilitate the understanding of the genetic and molecular mechanisms underlying the LJ trait. X. Li, C. Fang, M. Xu, F. Zhang, S. Lu, H. Nan, T. Su, S. Li, X. Zhao, L. Kong, X. Yuan, B. Liu, and F. Kong, The Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, No. 138 Haping Rd., Nangang District, Harbin 150081, China; S. Lu, H. Nan, X. Zhao, B. Liu, and F. Kong, School of Life Sciences, Guangzhou Univ., Guangzhou 510006, China; X. Li, C. Fang, F. Zhang, T. Su, S. Li, and L. Kong, Univ. of Chinese Academy of Sciences, Beijing 100049, China; X. Li, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Rd., Harbin 150086, China; J. Abe, Research Faculty of Agriculture, Hokkaido Univ., Sapporo, Hokkaido 060-8589, Japan; E.R. Cober, Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Bldg. #110, Central Experimental Farm, Ottawa, ON K1A 0C6, Canada. X. Li, C. Fang, M. Xu, F. Zhang and S. Lu contributed equally to this work. Received 18 Feb. 2017. Accepted 6 June 2017. *Corresponding author (kongfj@iga.ac.cn; elroy.cober@ agr.gc.ca; jabe@res.agr.hokudai.ac.jp; liubh@iga.ac.cn). Assigned to Associate Editor Zenglu Li. Abbreviations: CJ, conventional juvenile; DAE, days after emergence; HRM, high-resolution melting; Indel, insertion and deletion; LD, long day; LJ, long juvenile; LOD, logarithm of odds; QTL, quantitative trait locus; PCR, polymerase chain reaction; PGH, F2 population of the cross between Paranagoiana and Harosoy; PGI, F2 population of the cross between Paranagoiana and PI 159925; PGO, F2 population of the cross between Paranagoiana and OT94-47; SD, short day; SNP, singlenucleotide polymorphism; SSR, simple sequence repeat. Published in Crop Sci. 57:2547–2554 (2017). doi: 10.2135/cropsci2017.02.0106 © Crop Science Society of America | 5585 Guilford Rd., Madison, WI 53711 USA This is an open access article distributed under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Published online September 14, 2017